Optimization of Urea Based Protein Extraction from Formalin-Fixed Paraffin-Embedded Tissue for Shotgun Proteomics
Table 3
Summary of LC-ESI-MS/MS data when considering urea denaturation as a special factor in ProteinPilot. Detected peptides were limited to 1% global FDR. Detected proteins were limited to 2 peptides and 1% global FDR. Percent total carbamyl lysine was calculated as the number of total carbamyl groups out of the total number of peptides. Percent missed cleavages were calculated as the total number of missed cleavages out of the total number of peptides.