Analysis of High-Throughput Flow Cytometry Data Using plateCore
Figure 4
Plot showing the percentage of cells above the isotype threshold from plateCore (-axis) and FlowJo (-axis) for donor 1 (red) and 2 (blue) in channels FL1-H through FL4-H. plateCore gating for Phycoerythrin (PE) conjugated antibodies (FL2-H) was consistently lower than FlowJo, resulting in more cells above the isotype gate.