Keratinase Production by Three Bacillus spp. Using Feather Meal and Whole Feather as Substrate in a Submerged Fermentation

Figure 5

Cleavage of keratin substrate by extracellular peptidase activities of Bacillus sp. analysed by SDS-PAGE. K keratin; 1: supernatant control; 2: reaction mixture containing keratin and enzymatic extract before incubation; 3: reaction mixture after 1 hour of incubation at 37°C. Numbers on the left indicate relative molecular mass markers (kDa). Phosphorylase b (94 kDa), bovine serum albumin (67 kDa), ovalbumin (43 kDa), carbonic anhydrase (30 kDa), soybean trypsin inhibitor (20.1 kDa), and α-lactalbumin (14.4 kDa) were used as molecular mass standards.