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SI No. | Enzyme | Substrate | Reaction | Applications |
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1 | Oxidoreductase | | | |
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1.1 | Oxygenase | | | |
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1.1.1 | Monooxygenase | Alkane, steroids, fatty acid, and aromatic compounds | Incorporation of oxygen atom to substrate and utilize substrate as reducing agent. Desulfurization, dehalogenation, denitrification, ammonification, and hydroxylation of substrate | Protein engineering, bioremediation, synthetic chemistry, and so forth. |
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1.1.2 | Dioxygenase | Aromatic compounds | Introduction of two oxygen atom to the substrate results in intradiol cleaving and extradiol cleaving with the formation of aliphatic product | Synthetic chemistry, pharmaceutical industry, bioremediation, and so forth. |
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1.2 | Laccase | Ortho and paradiphenols, aminophenols, polyphenols, polyamines, lignins, and aryldiamines | Oxidation, decarboxylation and demethylation of substrate. | Food industry, paper and pulp industry, textile industry, nanotechnology, synthetic chemistry, bioremediation, cosmetics, and so forth. |
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1.3 | Peroxidase | | | |
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1.3.1 | Lignin peroxidase | Halogenated phenolic compounds, polycyclic aromatic compounds and other aromatic compounds | Oxidation of substrate in the presence of cosubstrate H2O2 and mediator like veratryl alcohol. | Food industry, paper and pulp industry, textile industry, pharmaceutical industry, bioremediation, and so forth. |
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1.3.2 | Manganese peroxidase | Lignin and other phenolic compounds | In the presence of Mn²+ and H2O2 the co-substrate catalyses oxidation of Mn²+ to Mn³+ which results in an Mn³+ chelateoxalate, which in turn oxidizes the phenolic substrates. | Food industry, Paper and pulp industry, textile industry, pharmaceutical industry, bioremediation, and so forth. |
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1.3.3 | Versatile peroxidase | Methoxybenzenes and phenolic aromatic | The enzyme catalyzes the electron transfer from an oxidizable substrate, with the formation and reduction of compound I and compound II intermediates. | Industrial biocatalyst, bioremediation, and so forth. |
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2 | Hydrolase | | | |
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2.1 | Lipase | Organic pollutants such as oil spill | The hydrolysis of triacylglycerols to glycerols and free-fatty acids | Control of oil spills, detergent production, baking industry, paper and pulp industry, personal care products, and so forth. |
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2.2 | Cellulase | Cellulosic substance | Hydrolyses the substrate to simple carbohydrates. | Textile manufacturing. detergent production, paper and pulp industry, bioremediation, and so forth. |
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2.3 | Protease | Proteins | Enzymes that hydrolyze peptide bonds in aqueous environment. | Leather, laundry, biocatalyst, bioremediation, and so forth. |
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