Sequence Analysis of Inducible Prophage phIS3501 Integrated into the Haemolysin II Gene of Bacillus thuringiensis var israelensis ATCC35646

Figure 1

Analysis of the chromosomal region containing phIS3501. (a) Bars on the top show the sequencing contigs from GenBank entries NZ_AAJM01000001–NZ_AAJM01000866, corresponding to the region encoding phIS3501. Unfilled parts correspond to bacterial sequences, grey parts correspond to the phage contigs detected using ERGOlight, in black are the GenBank contigs assigned to this phage during the sequencing proceeds. The unfilled bars on the bottom show the PCR products seen on the gel to the right, used for the sequence assembly verification. The numbers indicate gel lanes and the expected product sizes, corresponding to the following primer couples: (1) PHISB8 × PHISE8; (2) PHISC3 × PHISE5; (3) PHISB3 × PHISD7; (4) PHISD6 × PHISG8; (5) PHISA1 × PHISH2. A few molecular weight marker sizes (lane M) are indicated to the left of the gel. (b) Sequence-based genetic map of phIS3501. Similarity- or position-based identification of gene functions are shown by short descriptions of encoded proteins. Predicted protein coding genes correspond to the Entrez sequence acc. # JQ062992. In grey are the genes with hypothetical functions. Other colours correspond to the functional phage modules: red (p01, p04, p05 and p22) and yellow (p07, p09 and p21), lysogeny and lysogenic regulation, brown (p10 and p11), replication; dark green (p29–p32)—DNA packaging and maturation; green, blue and purple (p33–p36, p39, and p41–p43)—head and tail structural module; violet (p45 and p46), host lysis; and cyan (p50) indicates the FtsK family protein, probably involved in lysogenic recombination. Scale bar is in bp. (c) Contigs from Genbank entries NZ_AAJM01000001–NZ_AAJM01000866 revealed by BLASTN search against the completed phIS3501 sequence. The names of significant contigs with identities 80 to 94% are shown in italics. Other contigs, with the names shown in bold, have the identity of 97–99% and correspond to the phage. The entire phage sequence is represented on the top as the grey scale bar in bp.