CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering

Figure 4

Cas9-sgRNA-DNA complex. Cas9 protein is an architecture of multiple domains: RecI, RecII, Bridge helix, PAM-interacting, RuvC, and HNH, which accommodates negatively charged sgRNA-DNA heteroduplex. The complex triggers the cleavage of target DNA when sufficient RNA-DNA complementarity is available for the activation of HNH and RuvC nuclease domains.