Divergent Selection and Then What Not: The Conundrum of Missing Reproductive Isolation in Misty Lake and Stream Stickleback

Table 3

Ecotype main effect LS means ± S.E. and covariate slopes ± S.E. for head up, nest inspection, and nest entry in the Misty Lake (L), Inlet (I), and Outlet (O) three spine stickleback. The values are from final (reduced) covariate models in Table 1. For covariate effects, common slopes are given when there was no significant female ecotype×male trait interaction, whereas female ecotype specific slopes are given when these interactions were at least nearly significant. Models refer to models with (a) display and RSD, (b) display and ASD, (c) aggression and RSD, or (d) aggression and ASD as covariates in initial full model, with results of Tukey adjusted pairwise comparisons given below.

		Female response
Model		Head up	Nest inspection	Nest entry
(a) Display and RSD
Ecotype main effects
Female ecotype	Inlet	2.556±1.317	−0.926±0.458	−0.437±0.353
	Outlet	4.451±1.701	−1.905±0.591	−2.419±0.914
	Lake	0.945±0.821	−1.034±0.477	−2.349±0.841
Pairwise comparisons	I vs. L	ns	ns	°
	I vs. O	ns	ns	°
	L vs. O	°	ns	ns
Male ecotype	Inlet	0.728±1.076	−2.388±0.745	−3.651±1.318
	Outlet	3.550±1.471	−0.470±0.472	0.129±0.637
	Lake	3.682±1.724	−1.389±0.523	−1.684±0.755
Pairwise comparisons	I vs. L	ns	ns	ns
	I vs. O	ns	°	*
	L vs. O	ns	ns	ns
Slopes	Display	1.998±0.877 *	0.931±0.319 **	1.622±0.629 *
	RSD	−6.768±3.898 °	—	—
(b) Display and ASD
Ecotype main effects
Female ecotype	Inlet	0.706±0.512	Final model same as in a	Final model same as in a
	Outlet	4.122±1.247
	Lake	1.402±0.656
Pairwise comparisons	I vs. L	ns
	I vs. O	*
	L vs. O	*
Slopes	Display	1.373±0.486 **	4.977±1.711 **	Final model same as in a
(c) Aggression and RSD
Ecotype main effects
Female ecotype	Inlet	0.779±0.432	−0.587±0.365	−0.132±0.386
	Outlet	3.560±0.943	−2.029±0.709	−1.574±0.557
	Lake	0.632±0.454	−1.389±0.523	−2.423±0.696
Pairwise comparisons	I vs. L	ns	ns	ns
	I vs. O	*	ns	ns
	L vs. O	**	ns	°
Male ecotype	Inlet	2.863±0.773	−1.104±0.488	−1.091±0.519
	Outlet	1.154±0.574	−1.161±0.502	−0.661±0.480
	Lake	0.954±0.516	−1.741±0.542	−2.378±0.674
Pairwise comparisons	I vs. L	°	ns	*
	I vs. O	ns	ns	°
	L vs. O	ns	ns	ns
Slopes	Aggression	1.047±0.340 **		1.135±0.352 **
Female ecotype	Inlet	—	−0.035±0.436	—
	Outlet	—	1.817±0.787 *	—
	Lake	—	1.023±0.415 *	——
(d) Aggression and ASD
	Final models same as in c

P: °<0.1, *<0.05, **<0.01, ***<0.001.