Volatile Acid-Solvent Evaporation (VASE): Molecularly Homogeneous Distribution of Acyclovir in a Bioerodable Polymer Matrix for Long-Term Treatment of Herpes Simplex Virus-1 Infections

Figure 4

Release kinetics of ACV from SID-created v. VASE-created rods. Six 30:70 (w:w) ACV:PCL devices, created by the VASE method, and six 30:70 (w:w) ACV:PCL devices, created by the SID method were subjected to a 60-day trial examining release kinetics of ACV. Six VASE-created 0:100 (w:w) ACV:PCL devices and six SID-created 0:100 (w:w) ACV:PCL devices were used as a control. All devices were 7 mm 2 mm; two were used per well to ensure identical surface area to one 15 mm 2 mm device. Rods were placed in a 24-well plate and 1 mL DPBS was added. Each day for 60 days, that DPBS was removed and replaced. Each day’s collected solution was subjected to HPLC to determine ACV concentrations, which were graphed additively to show a stepwise increase in total ACV release over time. Right y-axis shows cumulative drug release from devices as a percent of total drug load.