Molecular Beacons: Powerful Tools for Imaging RNA in Living Cells

Table 1

Reporter-quencher pairs. For every one reporter is showed the peak of excitation, emission, and also the quenchers that could be used for observing a high quenching efficiency. The excitation peak and emission peak values were obtained from reference [58].

Reporter	Excitation peak (nm)	Emission peak (nm)	Compatible quenchers
Cy3	550	605	BHQ-2 and Dabcyl
Cy5	640	675	BHQ-3 and Dabcyl
CR-6G	505	570	Dabcyl
6-FAM	484	525	Iowa Black-FQ, BHQ-1 and Dabcyl
HEX	525	554	BHQ-1, Iowa Black-FQ and Dabcyl
TAMRA	555	605	Dabcyl
TET	490	535	BHQ-1, Iowa Black-FQ and Dabcyl