Selection of DNA Aptamers for Ovarian Cancer Biomarker CA125 Using One-Pot SELEX and High-Throughput Sequencing

Figure 2

Schematic representation of One-Pot SELEX. All steps occur in a single PCR tube. First the protein target (CA125) is allowed to adsorb to the inner surface of the tube. Multiple rinsing steps remove all proteins that are not strongly adsorbed. Then ssDNA—either unselected library or DNA selected in the previous SELEX round—is added and allowed to equilibrate with adsorbed protein. Vigorous rinsing retains only those DNA molecules that bind the protein target with high affinity. These DNA sequences are amplified via PCR in the final step.