Selection of DNA Aptamers for Ovarian Cancer Biomarker CA125 Using One-Pot SELEX and High-Throughput Sequencing

Figure 4

Flowchart showing the steps involved in analyzing high-throughput sequence data collected after a SELEX experiment. A series of sequence modification and counting steps is accomplished using Biopieces. Once tabulated based on abundance, the sequence information is analyzed using a locally written enrichment analysis script. CD-HIT is used to identify cluster sequences, and aptamers are chosen for characterization in vitro based on enrichment in response to the selective pressures of SELEX, their placement in a large sequence cluster, or both.