Selection of DNA Aptamers for Ovarian Cancer Biomarker CA125 Using One-Pot SELEX and High-Throughput Sequencing

Figure 5

Binding isotherm determined from fluorescence anisotropy of samples containing 100 nM Texas Red-labeled aptamer CA125_1 and varying concentrations of CA125. Fluorescence was excited with polarized light at 585 nm; emission at 635 nm was collected along optical paths parallel and perpendicular to the excitation light and used to calculate anisotropy.