A Sequence-Dependent DNA Condensation Induced by Prion Protein

Figure 5

Comparison of the effects of α-PrP (trace (a)) and C-terminal PrP 121–231 fragment (trace (b)) on the fluorescence property of YOYO intercalated in mDNA. and are the fluorescence intensities of the dye in the presence of DNA before and after the addition of protein, respectively, in 20 mM Hepes buffer, pH 7.4 containing 100 mM NaCl. Dye to DNA-phosphate 1 : 50. Excitation 485 nm; emission 505 nm. Temperature 20°C.