Identification and Expression of a Putative Facilitative Urea Transporter in Three Species of True Frogs (Ranidae): Implications for Terrestrial Adaptation

Figure 2

Phylogram illustrating the relatedness of novel UTs from Rana sylvatica, R. pipiens, and R. septentrionalis in comparison with known UTs from diverse taxa. This phylogram is a 50% MJ Rule Consensus Tree constructed from codon aligned nucleotides implementing a GTR+I evolutionary model. Numbers at branch points are Bayesian posterior probabilities; scale bars indicate number of expected changes per site. Inset: amphibian clade expanded for clarity. Accession numbers for nucleotide sequences are as follows: D. vulgaris, CP002297; A. japonica UT-C, AB181944; T. rubripes UT-C, AB181945; M. musculus UT-B, AF258602; H. sapiens UT-B1, BC050539; R. norvegicus UT-A4, AF042167; R. norvegicus UT-A3, AF041788; M. musculus UT-A3, AF258602; M. musculus UT-A2, AF367359; H. sapiens UT-A2, X96969; T. scripta elegans, AB308450; R. sylvatica, JF755889; R. pipiens, JF755890; R. septentrionalis, JF755891; R. esculenta, Y12784; B. marinus, AB212932; P. annectens, EU716115; O. beta, AF165893; T. rubripes, NM_001032724; A. japonica, AB049726; D. rerio, AY788989; T. scyllium, AB094993; S. acanthias, AF257331; D. say, AY277796; L. ocellata, AY052552.