Identification and Expression of a Putative Facilitative Urea Transporter in Three Species of True Frogs (Ranidae): Implications for Terrestrial Adaptation
Figure 3
Immunoblot of UT protein in bladder ( each) of R. sylvatica (A), R. pipiens (B), and R. septentrionalis (C) using an antibody (Ab) designed against the ranid UT. (A) In R. sylvatica, a band was primarily detected at 60 kDa, although several bands of higher mass were also detected. (B) Only a single band at 60 kDa was detected in R. pipiens. (C) In R. septentrionalis a faint band was detected at 60 kDa and at higher masses. (D) The band at 60 kDa in R. sylvatica was completely ablated in a competition assay in which the Ab was preincubated with a molar excess of its antigenic peptide; however, several bands of higher molecular mass remained. Only the bands at 60 kDa were analyzed; see text for details.
