Table of Contents
Advances in Biology
Volume 2014 (2014), Article ID 903259, 8 pages
http://dx.doi.org/10.1155/2014/903259
Research Article

The Role of TWIST in Angiogenesis and Cell Migration in Giant Cell Tumor of Bone

Department of Surgery, McMaster University, 711 Concession Street, B3 Surgical Offices, Hamilton, ON, Canada L8V 1C3

Received 13 February 2014; Revised 7 April 2014; Accepted 22 April 2014; Published 14 May 2014

Academic Editor: Julie Teruya-Feldstein

Copyright © 2014 Shalini Singh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Giant cell tumor of bone (GCT) is a bone tumor consisting of numerous multinucleated osteoclastic giant cells involved in bone resorption and neoplastic osteoblast-like stromal cells responsible for tumor growth. The tumor occasionally metastasizes to the lung; however, factors leading to metastasis in this tumor are unknown. The TWIST-1 protein (also referred to as TWIST) has been suggested to be involved in epithelial-mesenchymal transition (EMT) and tumor progression in some cancers. In this study we investigated the functional role of TWIST in GCT cell angiogenesis and migration. Overexpression of TWIST in neoplastic GCT stromal cells significantly increased mRNA and protein expression of VEGF and VEGFR1 in vitro, whereas knockdown of TWIST resulted in decreased VEGF and VEGFR1 expression. A stable cell line with TWIST overexpression resulted in features of EMT including increased cell migration and downregulation of E-cadherin. The results of our study indicate that TWIST may play an important role in angiogenesis and cell migration in GCT.