Amino Acid Starvation Enhances Programmed Ribosomal Frameshift in Metavirus Ty3 of Saccharomyces cerevisiae

Table 3

Gcn1p and Gcn20p are required for the increase of PRF efficiency in response to amino acid starvation.

Yeast strains and relevant mutations	% frameshift rates  ± SD
	Normal growth	3-AT treated
Wild type	4.0 ± 0.2	9.2 ± 0.5
(gcn1)	4.3 ± 0.1	4.4 ± 0.2
(gcn20)	4.4 ± 0.2	4.3 ± 0.4
(gcn1/gcn20)	4.7 ± 0.2	4.3 ± 0.5

% frameshift rates were calculated as the percent of the ratio of β-galactosidase activities expressed from the Ty3-frameshift plasmid to the β-galactosidase activities expressed from the Ty3-frame fusion plasmid in the relevant yeast transformants.
±SD: standard deviations.