Carbofuran Modulating Functions of Acetylcholinesterase from Rat Brain In Vitro

Table 1

Extraction of rat brain acetylcholinesterase.

Extraction buffer	Fractions	Total activity (mole/mL/min)	Total protein (mg)
Phosphate buffer (50 mM, pH 7.4)	Supernatant	0.165	7.75
	Pellet	0.562	3.05
Phosphate buffer with Triton X-100 (0.2% v/v) (50 mM, pH 7.4)	Supernatant	0.990	15.75
	Pellet	0.275	1.20

One unit of the activity of AChE has been defined as the micromoles of substrate hydrolyzed per min per mL.