FlowFP: A Bioconductor Package for Fingerprinting Flow Cytometric Data
Figure 1
FlowFP plot method to display gating data consistency. Fingerprints were computed using CD45 and SSC which are common variables in all tubes. Fingerprint similarity is indicated by color and in the similarity metric shown in each panel. The color wedge shows mapping of colors to values of the similarity metric (values above the maximum indicated on the wedge all map to red). The -axis for each subplot is fingerprint index, and the -axis is the log2 transformed fingerprint value plotted with zero at the center and scaled to ± “vertical scale factor” (in this case 3.0). (a) Sample FI05_000942, an example of a sample with good gating consistency. (b) Sample FI05_000599, an example of a sample with poor gating consistency. (c) and (d) as in (a) and (b), except that models were computed from Tube 1 only, rather than the aggregate of Tubes 1–7 for each sample. Note that the fingerprint for Tube 1 in both cases has zero deviation, as expected. Note also the qualitative similarity between (a) and (c) and between (b) and (d).
