Merging Mixture Components for Cell Population Identification in Flow Cytometry
Figure 2
Examples of the flowClust, flowClust, flowMerge cluster solutions for forward versus side scatter in a sample of CLL flow cytometry data. (a) The flowClust solution with seven clusters. (b) The flowClust solution with two clusters. (c) The entropy versus number of clusters plot, fit to a two-component piecewise linear regression model. The best fitting model has a changepoint at three clusters. (d) The flowMerge solution corresponding to clusters provides a better fit to the lymphocyte population than either the flowClust or flowClust solutions and provides a good estimate of the true number of cell populations.
