356141.fig.004a
(a)
356141.fig.004b
(b)
356141.fig.004c
(c)
356141.fig.004d
(d)
Figure 4: Plot showing the percentage of cells above the isotype threshold from plateCore ( -axis) and FlowJo ( -axis) for donor 1 (red) and 2 (blue) in channels FL1-H through FL4-H. plateCore gating for Phycoerythrin (PE) conjugated antibodies (FL2-H) was consistently lower than FlowJo, resulting in more cells above the isotype gate.