Inferring Biological Mechanisms by Data-Based Mathematical Modelling: Compartment-Specific Gene Activation during Sporulation in Bacillus subtilis as a Test Case
Figure 2
The regulation of the transcription factors during sporulation in Bacillus subtilis. (a) The solid lines show the concentration of RNA polymerase- holoenzyme before and after septation (time 0). Septation was modelled as a 4-fold increase in the concentration of the phosphatase IIE. The dashed lines show the model prediction if AB is not allosteric with both AA and binding with high affinity. (b) Predicted concentration of (lower curves) or (upper curves) RNA polymerase holoenzyme formed 90βmin after septation, as a function of an increase in IIE (including IIE complexed with AA-P) relative to the other sporulation proteins after 120βmin of protein expression, assuming that AB is allosteric (continuous lines) or not allosteric with both AA and binding with high affinity (dashed lines). (a) and (b) have been reproduced from [47].
