Figure 3: RMSD plots of the best (solid line) and worst (dashed line) sequences ranked by ERIS after 1000 generation (a) and 2000 generations (b), respectively. Models after 20 ns MD simulations were aligned to the wild-type structure of the Z domain using residues 6–17 and 22–33 (Helix 1 and 2). 𝐶 𝛼 RMSD of Helix 3 (residue 39–53) was calculated and smoothed using spline interpolation.