An Integrated Framework to Model Cellular Phenotype as a Component of Biochemical Networks

Figure 3

In silico gene knock-down models predict the viability of synthetic lethal and synthetic rescue double-gene knock-out experiments. The outcomes of double-gene knock-out experiments were obtained from publications compiled by the Saccharomyces Genome Database [20]. Pairs of genes that form synthetic phenotypes are identified by the color of the squares at the intersection of rows and columns. Experimentally observed synthetic lethal and synthetic rescue mutations are indicated by red and blue squares, respectively. The predicted outcome of double-gene knockouts is indicated by the pattern of the squares. Correct and incorrect predictions are marked with filled and diagonally hashed squares, respectively. Seven out of thirteen synthetic lethal and two out of five synthetic rescue phenotypes are correctly predicted.