Structural Basis for Species Selectivity in the HIV-1 gp120-CD4 Interaction: Restoring Affinity to gp120 in Murine CD4 Mimetic Peptides

Table 2

Sequences of CD4 mimetic peptides. hCD4-M and mCD4-M represent the wild-type mimetic peptides presenting residues 22–64 of hCD4 and 22–66 of mCD4, respectively. Residues of hCD4-M and mCD4-M that were replaced by alanine in hCD4-M* and mCD4-M*, respectively, are shown in bold. mCD4-M** represents a cyclic peptide that was proposed based on the computational simulations. The position of the engineered disulfide bond is indicated by brackets next to the involved cysteine residues.

Peptide	Sequence
hCD4-M	Ac-C-Ahx*-^22KSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQ^64-NH2
	Ac-HHHHHH-Ahx-KSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQ-NH2
hCD4-M*	Ac-C-Ahx-KSIQFHWKNSNQIKILGNQGSALTKGPSKLNDRADSAASLWDQ-NH2
mCD4-M	Ac-C-Ahx-^22KITVFTWKFSDQRKILGQHGKGVLIRGGSPSQFDRFDSKKGAWEK^66-NH2
mCD4-M*	Ac-C-Ahx-KITVFTWKFSDQRKILGQHGKGALIRGGSPSQFDRFDSAAGAWEK-NH2
mCD4-M**	Ac-HHHHHH-Ahx-K [CTVFTWKFSDQRKILGQHGKGVLIRGGSPSQFDRFDSKKGAWC]K- NH2

*Ahx: ε-aminohexanoic acid (spacer between tag and peptide sequence).