Flux Analysis of the Trypanosoma brucei Glycolysis Based on a Multiobjective-Criteria Bioinformatic Approach
Figure 8
Comparison of the prediction performance between FBA-SimVis and Metaboflux. The flux proportion is given by the edge size, circles stands for metabolites, and square for enzymes. (a) Flux map obtained by FBA-SimVis. (b) Flux map obtained by Metaboflux using a single objective function. (c) Flux map obtained by Metaboflux with additional constraints on balance states and experimental knowledge. (d) Histogram that gives the expected proportion (considering experimental data), and proportions obtained with Metaboflux using the FBA-SimVis flux results as constraints and Metaboflux. The metabolite biomasses were deduced from the number tokens given by Metaboflux. Deduced from empirical measurements, the glucose biomass was set to 1000 tokens and ATP, ADP, NADH, and NAD+ were set to 2000. Experimental data given as ratios of excreted metabolites were used to specify expected number of tokens. The balance ratio of ATP, ADP, NADH, and NAD+ was specified by an equality equation.
