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Advances in Bioinformatics
Volume 2014, Article ID 101894, 8 pages
http://dx.doi.org/10.1155/2014/101894
Research Article

Multiplex Degenerate Primer Design for Targeted Whole Genome Amplification of Many Viral Genomes

1Computations, Lawrence Livermore National Laboratory (LLNL), Livermore, CA 94550, USA
2Physical and Life Sciences/Global Security, Lawrence Livermore National Laboratory (LLNL), Livermore, CA 94550, USA

Received 30 May 2014; Accepted 14 July 2014; Published 3 August 2014

Academic Editor: Paul Harrison

Copyright © 2014 Shea N. Gardner et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Citations to this Article [4 citations]

The following is the list of published articles that have cited the current article.

  • Mathijs, Vandenbussche, and Van Borm, “Using genomics for surveillance of veterinary infectious agents,” OIE Revue Scientifique et Technique, vol. 35, no. 1, pp. 143–157, 2016. View at Publisher · View at Google Scholar
  • Monica K. Borucki, Victoria Lao, Mona Hwang, Shea Gardner, Danielle Adney, Vincent Munster, Richard Bowen, and Jonathan E. Allen, “Middle East Respiratory Syndrome Coronavirus Intra-Host Populations Are Characterized by Numerous High Frequency Variants,” Plos One, vol. 11, no. 1, 2016. View at Publisher · View at Google Scholar
  • Ruslan Kalendar, Bekbolat Khassenov, Yerlan Ramankulov, Olga Samuilova, and Konstantin I. Ivanov, “FastPCR: An in silico tool for fast primer and probe design and advanced sequence analysis,” Genomics, 2017. View at Publisher · View at Google Scholar
  • Joshua Quick, Nathan D Grubaugh, Steven T Pullan, Ingra M Claro, Andrew D Smith, Karthik Gangavarapu, Glenn Oliveira, Refugio Robles-Sikisaka, Thomas F Rogers, Nathan A Beutler, Dennis R Burton, Lia Laura Lewis-Ximenez, Jaqueline Goes de Jesus, Marta Giovanetti, Sarah C Hill, Allison Black, Trevor Bedford, Miles W Carroll, Marcio Nunes, Luiz Carlos Alcantara, Ester C Sabino, Sally A Baylis, Nuno R Faria, Matthew Loose, Jared T Simpson, Oliver G Pybus, Kristian G Andersen, and Nicholas J Loman, “Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples,” Nature Protocols, vol. 12, no. 6, pp. 1261–1276, 2017. View at Publisher · View at Google Scholar