Elementary Flux Mode Analysis of Acetyl-CoA Pathway in Carboxydothermus hydrogenoformans Z-2901

Table 1

Reactions and enzymes involved in the acetyl-CoA pathway model of C. hydrogenoformans.

Reaction number	Reactions	Gene name	EC number	Enzyme
R 1:	pyruvate intake	*	*	Influx
R 2:	⟹ CO	*	*	Influx
R 3:	malate ⟹	#	#	Efflux
R 4:	OAA ⟹	#	#	Efflux
R 5:	pyr ⟹ CO2 + ACoA	aceE	1.2.4.1	Pyruvate dehydrogenase E1
R 6:	pyr + CO2 ⟺ malate	maeA	1.1.1.38	Malic enzyme
R 7:	OAA + NADH ⟺ malate	mdh1	1.1.1.37	Malate dehydrogenase
R 8:	pyr + ATP ⟹ PEP	ppdK	2.7.9.1	Pyruvate phosphate dikinase
R 9:	PEP ⟹ pyr + ATP	pyk	2.7.1.40	Pyruvate kinase
R 10:	CO2 + PEP ⟺ OAA	pepC	4.1.1.31	PEP carboxylase
R 11:	pyr + CO2 ⟹ OAA	pykA	6.4.1.1	Pyruvate kinase II
R 12:	OAA ⟹ pyr + ATP	oadA	4.1.1.3	Pyruvate carboxylase subunit
R 13:	ACoA ⟺ acetylP	pta	2.3.1.8	Phosphate acetyltransferase
R 14:	acetylP ⟺ ATP + acetate	ackA	2.7.2.1	Acetate kinase
R 15:	ACoA + H2O ⟹ malate	glcB	2.3.3.9	Malate synthase G
R 16:	ACoA ⟺ ATP + acetate	acdA	6.2.1.13	Acetyl-CoA synthase
R 17:	acetaldehyde ⟺ ACoA	mhpF	1.2.1.10	Acetaldehyde dehydrogenase
R 18:	NADH + acetate ⟺ H2O + acetaldehyde	acsA	6.2.1.1	Acetyl-coenzyme A synthetase
R 19:	ACoA ⟹ CO	acsB	2.3.1.169	CO-methylating acetyl-CoA synthase
R 20:	H2O + CO ⟹ CO2 + H2	cooSV	1.2.99.2	Carbon-monoxide dehydrogenase
R 21:	Formate + NAD^+⟹ CO2 + NADH	fdoG	1.2.1.2	Formate dehydrogenase-O
R 22:	CO2 + MFR ⟹ H2O + fMFR	fwd	1.2.99.5	Tungsten formylmethanofuran dehydrogenase subunit E
R 23:	fMFR + H2O ⟹ Formate + MFR	fmdE	1.2.99.5	Formylmethanofuran dehydrogenase
R 24:	2 H^+⟹ H2	hydA	1.12.7.2	Ni/Fe hydrogenase large subunit
R 25:	H2 ⟹	#	#	Efflux
R 26:	Acetate ⟹	#	#	Efflux

 *Substrate influx into the system.
^#External product efflux from the system.