Research Article

PhosphoHunter: An Efficient Software Tool for Phosphopeptide Identification

Table 4

Performance of PhosphoHunter in terms of sequence detection and phosphorylation sites localization.

Intensity thresholdSequences identifiedSequences identified
(all the hits)(hits with -value ≤  )
Correct sequencesCorrect sitesCorrect sequencesCorrect sites


The 750 MS/MS spectra of phosphorylated peptides were considered and the intensity threshold was tuned from 0 to 100.