Systematic Bioinformatic Approach for Prediction of Linear B-Cell Epitopes on Dengue E and prM Protein

Table 2

Epitopes predicted by bioinformatics on prM protein with conservancy results.

ID of the predicted epitope	^#Epitope sequence	Epitope length (a.a.)	Method of prediction (score)	Percentage conservancy (min%)
				Intraserotype				Pan-serotype
				DENV1	DENV2	DENV3	DENV4
Entire prM protein sequence				93	87	98	96	63
EP1/prM	FHLTTRGGE; 1–9	9	Ellipro (0.6)	88	77	88	88	55
EP2/prM	TTRGGEPHMIVSKQERG; 4–20	17	BepiPred (0.05–1.1)	88	71	94	94	59
EP3/prM	SKQERGKS; 15–22	8	Ellipro (0.5)	87	37	87	87	25
EP4/prM	KTAEG; 26–30	5	Ellipro (0.5)	80	60	100	100	60
EP5/prM	IAMDL; 37–41	5	Ellipro (0.5)	100	40	100	80	40
EP6/prM	LCEDTMTYKCPRITEAEPDDVDCWCNATDTWVTYGTCSQTGEHRRDKRSV; 44–93	50	BepiPred (−0.02–1.7)	82	92	96	96	68
EP7/prM	RITEAEPDDVD; 55–65	11	Ellipro (0.6)	36	81	81	90	18
EP8/prM	VALAPHVGLGLETRTETWMS; 93–112	20	SVMTriP20 (1.0)	95	100	95	90	75
EP9/prM	LETRTETWMSSEGAWKQIQKV; 103–123	21	BepiPred (−0.04–0.8)	90	95	95	95	57
EP10/prM	TWALR; 125–129	5	BepiPred (−0.09–0.1)	80	80	80	100	60
EP11/prM	ALRHPGFTIALFLAHAIGT; 127–146	20	SVMTriP20 (0.8)	90	95	85	90	50
EP12/prM	GAWKQIQRVETWALRHPGFTVILALFLAH AIGTSITQKGIIFILLMLVTPS; 115–165	50	Ellipro (0.7)	86	80	94	94	46
EP13/prM	GTSITQ; 145–150	6	BepiPred (−0.06–0.4)	100	83	83	100	33

Sequence position. Epitope sequence is given with reference to DENV1 isolate AY713476. Epitopes that have been previously described by other authors (cited in the Discussion).