Table 2: Epitopes predicted by bioinformatics on prM protein with conservancy results.

ID of the predicted epitope#Epitope sequenceEpitope length (a.a.)Method of prediction (score)Percentage conservancy (min%)
IntraserotypePan-serotype
DENV1DENV2DENV3DENV4

Entire prM protein sequence9387989663
EP1/prMFHLTTRGGE
1–9
9Ellipro (0.6)8877888855
EP2/prMTTRGGEPHMIVSKQERG
4–20
17BepiPred (0.05–1.1)8871949459
EP3/prMSKQERGKS
15–22
8Ellipro (0.5)8737878725
EP4/prMKTAEG
26–30
5Ellipro (0.5)806010010060
EP5/prMIAMDL
37–41
5Ellipro (0.5)100401008040
EP6/prMLCEDTMTYKCPRITEAEPDDVDCWCNATDTWVTYGTCSQTGEHRRDKRSV
44–93
50BepiPred (−0.02–1.7)8292969668
EP7/prMRITEAEPDDVD
55–65
11Ellipro (0.6)3681819018
EP8/prMVALAPHVGLGLETRTETWMS
93–112
20SVMTriP20 (1.0)95100959075
EP9/prM LETRTETWMSSEGAWKQIQKV
103–123
21BepiPred (−0.04–0.8)9095959557
EP10/prMTWALR
125–129
5BepiPred (−0.09–0.1)80808010060
EP11/prMALRHPGFTIALFLAHAIGT
127–146
20SVMTriP20 (0.8)9095859050
EP12/prMGAWKQIQRVETWALRHPGFTVILALFLAH AIGTSITQKGIIFILLMLVTPS
115–165
50Ellipro (0.7)8680949446
EP13/prMGTSITQ
145–150
6BepiPred (−0.06–0.4)100838310033

Sequence position. Epitope sequence is given with reference to DENV1 isolate AY713476. Epitopes that have been previously described by other authors (cited in the Discussion).