Research Article
Immunoinformatics Approach for Multiepitopes Vaccine Prediction against Glycoprotein B of Avian Infectious Laryngotracheitis Virus
Table 4
List of best six epitopes that bind with high affinity with the human MHC class II alleles.
| | Core Sequence | Peptide Sequence | Start | End | Allele | IC50 |
| | LLRSTVSKA | LPLVPSLLRSTVSKA | 192 | 206 | HLA-DQA101:02/DQB106:02 | 824.7 | | HLA-DRB101:01 | 99.4 | | HLA-DRB103:01 | 421.6 | | HLA-DRB104:01 | 300.9 | | HLA-DRB104:05 | 768.9 | | HLA-DRB107:01 | 155.6 | | HLA-DRB108:02 | 143.9 | | HLA-DRB109:01 | 783.8 | | HLA-DRB115:01 | 888.9 | | PLVPSLLRSTVSKAF | 193 | 207 | HLA-DPA102:01/DPB101:01 | 436 | | HLA-DQA101:02/DQB106:02 | 538.2 | | HLA-DQA105:01/DQB103:01 | 413.8 | | HLA-DRB101:01 | 42.1 | | HLA-DRB103:01 | 123.5 | | HLA-DRB104:01 | 185.5 | | HLA-DRB104:04 | 166.7 | | HLA-DRB104:05 | 478 | | HLA-DRB108:02 | 103 | | HLA-DRB109:01 | 394.9 | | HLA-DRB113:02 | 774.7 | | HLA-DRB115:01 | 978 | | LVPSLLRSTVSKAFH | 194 | 208 | HLA-DPA102:01/DPB101:01 | 464.3 | | HLA-DQA101:02/DQB106:02 | 457.4 | | HLA-DQA105:01/DQB103:01 | 378.5 | | HLA-DRB101:01 | 26.9 | | HLA-DRB103:01 | 57.1 | | HLA-DRB104:01 | 147.6 | | HLA-DRB104:05 | 360 | | HLA-DRB108:02 | 71.5 | | HLA-DRB109:01 | 336.5 | | HLA-DRB111:01 | 148.8 | | HLA-DRB113:02 | 652.3 | | HLA-DRB115:01 | 824.6 | | VPSLLRSTVSKAFHT | 195 | 209 | HLA-DPA102:01/DPB101:01 | 682.2 | | HLA-DQA101:02/DQB106:02 | 397.2 | | HLA-DRB101:01 | 18 | | HLA-DRB103:01 | 32.8 | | HLA-DRB104:01 | 108.8 | | HLA-DRB104:05 | 303.1 | | HLA-DRB108:02 | 52 | | HLA-DRB109:01 | 219.9 | | HLA-DRB113:02 | 568.1 | | HLA-DRB115:01 | 745 | | PSLLRSTVSKAFHTT | 196 | 210 | HLA-DPA102:01/DPB101:01 | 871.8 | | HLA-DQA101:02/DQB106:02 | 506.4 | | HLA-DRB101:01 | 26.3 | | HLA-DRB103:01 | 49.1 | | HLA-DRB104:01 | 126.7 | | HLA-DRB104:04 | 178.1 | | HLA-DRB104:05 | 369.2 | | HLA-DRB108:02 | 61.5 | | HLA-DRB115:01 | 809.3 | | SLLRSTVSKAFHTTN | 197 | 211 | HLA-DQA101:02/DQB106:02 | 609.2 | | HLA-DRB101:01 | 36.6 | | HLA-DRB103:01 | 89.2 | | HLA-DRB104:01 | 130.9 | | HLA-DRB104:05 | 467.4 | | HLA-DRB108:02 | 75.6 | | LLRSTVSKAFHTTNF | 198 | 212 | HLA-DQA101:02/DQB106:02 | 836.1 | | HLA-DRB103:01 | 218.7 | | HLA-DRB104:01 | 184.1 | | HLA-DRB104:04 | 384.5 | | HLA-DRB104:05 | 643.2 | | HLA-DRB108:02 | 106 |
| | FLEIANYQV | VYRDYRFLEIANYQV
| 271
| 285
| HLA-DRB107:01 | 9.1 | | HLA-DRB108:02 | 752 | | HLA-DRB113:02 | 288.3 | | HLA-DRB501:01 | 67.4 | YRDYRFLEIANYQVR
| 272
| 286
| HLA-DRB107:01 | 10.9 | | HLA-DRB108:02 | 565 | | HLA-DRB113:02 | 237.5 | | HLA-DRB501:01 | 29.8 | | RDYRFLEIANYQVRD | 273 | 287 | HLA-DPA101/DPB104:01 | 284.1 | | HLA-DPA101:03/DPB102:01 | 90.8 | | HLA-DRB101:01 | 8.9 | | HLA-DRB104:01 | 41.8 | | HLA-DRB107:01 | 15.6 | | HLA-DRB108:02 | 585.2 | | HLA-DRB111:01 | 54.1 | | HLA-DRB113:02 | 216.2 | | HLA-DRB301:01 | 875 | | HLA-DRB501:01 | 27.2 | | HLA-DPA102:01/DPB105:01 | 218.5 | | HLA-DQA101:01/DQB105:01 | 637.4 | | HLA-DQA101:02/DQB106:02 | 331.2 | | HLA-DRB104:04 | 81.1 | | HLA-DRB104:05 | 32.1 | | HLA-DRB109:01 | 273.4 | | DYRFLEIANYQVRDL | 274 | 288 | HLA-DPA101/DPB104:01 | 475 | | HLA-DPA101:03/DPB102:01 | 72.5 | | HLA-DPA102:01/DPB101:01 | 42.9 | | HLA-DPA102:01/DPB105:01 | 294.4 | | HLA-DPA103:01/DPB104:02 | 462.5 | | HLA-DRB101:01 | 8.3 | | HLA-DRB104:01 | 58.2 | | HLA-DRB104:05 | 39.5 | | HLA-DRB107:01 | 15.9 | | HLA-DRB108:02 | 704.3 | | HLA-DRB111:01 | 43.5 | | HLA-DRB113:02 | 190.3 | | HLA-DRB301:01 | 979.2 | | HLA-DRB501:01 | 18.3 | | RFLEIANYQVRDLET | 276 | 290 | HLA-DPA101/DPB104:01 | 594 | | HLA-DPA101:03/DPB102:01 | 113.7 | | HLA-DPA102:01/DPB101:01 | 66 | | HLA-DPA102:01/DPB105:01 | 726.4 | | HLA-DRB101:01 | 15.5 | | HLA-DRB104:01 | 261.2 | | HLA-DRB104:04 | 174.2 | | HLA-DRB104:05 | 174.3 | | HLA-DRB107:01 | 31 | | HLA-DRB109:01 | 931.6 | | HLA-DRB111:01 | 140.6 | | HLA-DRB113:02 | 486.4 | | HLA-DRB501:01 | 39 | | FLEIANYQVRDLETG | 277 | 291 | HLA-DPA101:03/DPB102:01 | 336.8 | | HLA-DPA102:01/DPB101:01 | 146.9 | | HLA-DRB101:01 | 28.9 | | HLA-DRB104:04 | 273.3 | | HLA-DRB104:05 | 325.2 | | HLA-DRB107:01 | 57.2 | | HLA-DRB111:01 | 340.9 | | HLA-DRB113:02 | 852.9 | | HLA-DRB501:01 | 101.7 |
| | FLTDEQFTI | PPKKRNFLTDEQFTI | 295 | 309 | HLA-DPA101/DPB104:01 | 246.4 | | HLA-DPA101:03/DPB102:01 | 35.5 | | HLA-DPA102:01/DPB101:01 | 93.6 | | HLA-DPA102:01/DPB105:01 | 541 | | HLA-DPA103:01/DPB104:02 | 506.6 | | HLA-DRB103:01 | 76.1 | | HLA-DRB104:01 | 198.7 | | HLA-DRB104:05 | 274.9 | | HLA-DRB107:01 | 98.6 | | HLA-DRB301:01 | 4.9 | | HLA-DRB501:01 | 948.8 | | PKKRNFLTDEQFTIG | 296 | 310 | HLA-DPA101/DPB104:01 | 172.7 | | HLA-DPA101:03/DPB102:01 | 24.5 | | HLA-DPA102:01/DPB101:01 | 82.3 | | HLA-DPA102:01/DPB105:01 | 475.5 | | HLA-DPA103:01/DPB104:02 | 341.5 | | HLA-DRB101:01 | 850.7 | | HLA-DRB103:01 | 59.9 | | HLA-DRB104:01 | 136.1 | | HLA-DRB104:04 | 869.9 | | HLA-DRB104:05 | 325 | | HLA-DRB107:01 | 162 | | HLA-DRB301:01 | 4.8 | | HLA-DRB501:01 | 769 | | KKRNFLTDEQFTIGW | 297 | 311 | HLA-DPA101/DPB104:01 | 123 | | HLA-DPA101:03/DPB102:01 | 20.1 | | HLA-DPA102:01/DPB101:01 | 66.4 | | HLA-DPA102:01/DPB105:01 | 394.6 | | HLA-DPA103:01/DPB104:02 | 199 | | HLA-DRB101:01 | 342.9 | | HLA-DRB103:01 | 37.7 | | HLA-DRB104:01 | 103.7 | | HLA-DRB104:04 | 805.9 | | HLA-DRB104:05 | 330.3 | | HLA-DRB107:01 | 203.9 | | HLA-DRB301:01 | 4.6 | | HLA-DRB501:01 | 527 | | KRNFLTDEQFTIGWD | 298 | 312 | HLA-DPA101/DPB104:01 | 110.8 | | HLA-DPA101:03/DPB102:01 | 21.6 | | HLA-DPA102:01/DPB101:01 | 65 | | HLA-DPA102:01/DPB105:01 | 405.2 | | HLA-DPA103:01/DPB104:02 | 148.2 | | HLA-DRB101:01 | 354.3 | | HLA-DRB103:01 | 31.7 | | HLA-DRB104:01 | 98.2 | | HLA-DRB104:05 | 398.7 | | HLA-DRB107:01 | 325.7 | | HLA-DRB301:01 | 4.4 | | HLA-DRB501:01 | 505.9 | | RNFLTDEQFTIGWDA | 299 | 313 | HLA-DPA101/DPB104:01 | 115.3 | | HLA-DPA101:03/DPB102:01 | 26.7 | | HLA-DPA102:01/DPB101:01 | 88.5 | | HLA-DPA102:01/DPB105:01 | 532.9 | | HLA-DPA103:01/DPB104:02 | 182.5 | | HLA-DRB101:01 | 687.7 | | HLA-DRB103:01 | 64.7 | | HLA-DRB104:01 | 178.3 | | HLA-DRB104:05 | 700.1 | | HLA-DRB301:01 | 5.9 | | HLA-DRB501:01 | 948.3 | | NFLTDEQFTIGWDAM | 300 | 314 | HLA-DPA101/DPB104:01 | 244 | | HLA-DPA101:03/DPB102:01 | 44.6 | | HLA-DPA102:01/DPB101:01 | 173.3 | | HLA-DPA103:01/DPB104:02 | 250.2 | | HLA-DQA105:01/DQB102:01 | 475.1 | | HLA-DRB103:01 | 164.3 | | HLA-DRB104:01 | 278.3 | | HLA-DRB301:01 | 9 | | FLTDEQFTIGWDAME | 301 | 315 | HLA-DPA101/DPB104:01 | 640 | | HLA-DPA101:03/DPB102:01 | 154.8 | | HLA-DPA102:01/DPB101:01 | 395.6 | | HLA-DPA103:01/DPB104:02 | 437.8 | | HLA-DQA105:01/DQB102:01 | 459.5 | | HLA-DRB103:01 | 433.9 | | HLA-DRB104:01 | 271.4 | | HLA-DRB301:01 | 15.5 |
| | LLGDIVAVS | QPVSARLLGDIVAVS | 519 | 533 | HLA-DPA102:01/DPB101:01 | 922.5 | | HLA-DPA103:01/DPB104:02 | 822.3 | | HLA-DQA101:02/DQB106:02 | 726 | | HLA-DQA105:01/DQB103:01 | 42.2 | | HLA-DRB104:01 | 494.1 | | HLA-DRB108:02 | 914.5 | | HLA-DRB301:01 | 211.5 | | PVSARLLGDIVAVSK | 520 | 534 | HLA-DPA102:01/DPB101:01 | 752.7 | | HLA-DPA103:01/DPB104:02 | 853.4 | | HLA-DQA101:02/DQB106:02 | 715.4 | | HLA-DQA105:01/DQB103:01 | 34 | | HLA-DRB103:01 | 380.3 | | HLA-DRB104:01 | 298 | | HLA-DRB104:05 | 458.8 | | HLA-DRB108:02 | 571.8 | | HLA-DRB301:01 | 196.5 | | VSARLLGDIVAVSKC | 521 | 535 | HLA-DPA102:01/DPB101:01 | 723.1 | | HLA-DPA103:01/DPB104:02 | 733.4 | | HLA-DQA101:02/DQB106:02 | 502.8 | | HLA-DQA105:01/DQB103:01 | 29.9 | | HLA-DRB103:01 | 214.3 | | HLA-DRB104:01 | 237 | | HLA-DRB104:05 | 429.4 | | HLA-DRB108:02 | 541.8 | | HLA-DRB301:01 | 172.3 | | SARLLGDIVAVSKCI | 522 | 536 | HLA-DPA102:01/DPB101:01 | 677.6 | | HLA-DPA103:01/DPB104:02 | 650.5 | | HLA-DQA101:02/DQB106:02 | 555.2 | | HLA-DQA105:01/DQB103:01 | 26.4 | | HLA-DRB101:01 | 175.3 | | HLA-DRB103:01 | 124.9 | | HLA-DRB104:01 | 187.9 | | HLA-DRB104:05 | 396.3 | | HLA-DRB108:02 | 396.7 | | HLA-DRB301:01 | 143.4 | | ARLLGDIVAVSKCIE | 523 | 537 | HLA-DPA102:01/DPB101:01 | 791.2 | | HLA-DPA103:01/DPB104:02 | 787.4 | | HLA-DQA101:02/DQB106:02 | 590.2 | | HLA-DQA105:01/DQB103:01 | 31.5 | | HLA-DRB101:01 | 270.9 | | HLA-DRB103:01 | 199.3 | | HLA-DRB104:01 | 255.3 | | HLA-DRB104:05 | 643.6 | | HLA-DRB108:02 | 360.5 | | HLA-DRB301:01 | 379.2 | | RLLGDIVAVSKCIEI | 524 | 538 | HLA-DPA102:01/DPB101:01 | 807.2 | | HLA-DPA103:01/DPB104:02 | 911.1 | | HLA-DQA101:02/DQB106:02 | 621.6 | | HLA-DQA105:01/DQB103:01 | 35.4 | | HLA-DRB103:01 | 347.3 | | HLA-DRB104:01 | 375.6 | | HLA-DRB104:05 | 977.9 | | HLA-DRB108:02 | 414.9 | | HLA-DRB301:01 | 974.2 | | LLGDIVAVSKCIEIP | 525 | 539 | HLA-DPA102:01/DPB101:01 | 941 | | HLA-DQA101:02/DQB106:02 | 748.6 | | HLA-DQA105:01/DQB103:01 | 37.1 | | HLA-DRB103:01 | 688 | | HLA-DRB104:01 | 848.5 | | HLA-DRB108:02 | 521 |
| | IASFLSNPF | ISTVSGIASFLSNPF | 737 | 751 | HLA-DPA102:01/DPB101:01 | 264.9 | | HLA-DQA105:01/DQB102:01 | 384.2 | | HLA-DRB104:01 | 53.5 | | HLA-DRB104:04 | 92.5 | | HLA-DRB104:05 | 16.2 | | HLA-DRB107:01 | 25 | | HLA-DRB109:01 | 104.9 | | HLA-DRB115:01 | 24.4 | | HLA-DRB501:01 | 103.5 | | STVSGIASFLSNPFA | 738 | 752 | HLA-DPA101/DPB104:01 | 559.3 | | HLA-DPA101:03/DPB102:01 | 320.6 | | HLA-DPA102:01/DPB101:01 | 240.5 | | HLA-DQA101:01/DQB105:01 | 976.4 | | HLA-DQA105:01/DQB102:01 | 474.6 | | HLA-DRB101:01 | 101.3 | | HLA-DRB104:01 | 41.8 | | HLA-DRB104:05 | 15.5 | | HLA-DRB107:01 | 31.3 | | HLA-DRB108:02 | 589.8 | | HLA-DRB109:01 | 74.3 | | HLA-DRB115:01 | 18.9 | | HLA-DRB501:01 | 77.6 | | TVSGIASFLSNPFAA | 739 | 753 | HLA-DPA101:03/DPB102:01 | 179.9 | | HLA-DPA102:01/DPB101:01 | 219.5 | | HLA-DQA101:01/DQB105:01 | 915.8 | | HLA-DQA105:01/DQB102:01 | 611.8 | | HLA-DRB101:01 | 40.6 | | HLA-DRB104:01 | 27.7 | | HLA-DRB104:05 | 15.5 | | HLA-DRB107:01 | 44 | | HLA-DRB108:02 | 538.8 | | HLA-DRB109:01 | 72.2 | | HLA-DRB115:01 | 15.9 | | HLA-DRB501:01 | 57.1 | | VSGIASFLSNPFAAL | 740 | 754 | HLA-DQA101:01/DQB105:01 | 710 | | HLA-DQA105:01/DQB102:01 | 755.5 | | HLA-DRB101:01 | 14.2 | | HLA-DRB103:01 | 427.9 | | HLA-DRB104:01 | 22 | | HLA-DRB104:05 | 14.6 | | HLA-DRB107:01 | 43.3 | | HLA-DRB108:02 | 486.4 | | HLA-DRB109:01 | 52.2 | | HLA-DRB111:01 | 377.7 | | HLA-DRB115:01 | 12.8 | | HLA-DRB501:01 | 29.4 | | SGIASFLSNPFAALG | 741 | 755 | HLA-DQA101:01/DQB105:01 | 936.1 | | HLA-DRB103:01 | 629.7 | | HLA-DRB104:01 | 27.6 | | HLA-DRB104:05 | 18 | | HLA-DRB107:01 | 67.9 | | HLA-DRB108:02 | 582.5 | | HLA-DRB109:01 | 56.2 | | HLA-DRB115:01 | 14.6 | | HLA-DRB501:01 | 50.2 | | GIASFLSNPFAALGI | 742 | 756 | HLA-DRB101:01 | 27.1 | | HLA-DRB104:01 | 38.1 | | HLA-DRB104:05 | 27.3 | | HLA-DRB108:02 | 594.2 | | HLA-DRB109:01 | 58.6 | | HLA-DRB115:01 | 17.4 | | HLA-DRB501:01 | 73.5 | | IASFLSNPFAALGIG | 743 | 757 | HLA-DRB104:01 | 67 | | HLA-DRB104:05 | 41.9 | | HLA-DRB115:01 | 28.6 | | HLA-DRB401:01 | 735.5 | | HLA-DRB501:01 | 119.8 |
| | LLGDIVAVS | QPVSARLLGDIVAVS | 519 | 533 | HLA-DPA102:01/DPB101:01 | 922.5 | | HLA-DPA103:01/DPB104:02 | 822.3 | | HLA-DQA101:02/DQB106:02 | 726 | | HLA-DQA105:01/DQB103:01 | 42.2 | | HLA-DRB104:01 | 494.1 | | HLA-DRB108:02 | 914.5 | | HLA-DRB301:01 | 211.5 | | PVSARLLGDIVAVSK | 520 | 534 | HLA-DPA102:01/DPB101:01 | 752.7 | | HLA-DPA103:01/DPB104:02 | 853.4 | | HLA-DQA101:02/DQB106:02 | 715.4 | | HLA-DQA105:01/DQB103:01 | 34 | | HLA-DRB103:01 | 380.3 | | HLA-DRB104:01 | 298 | | HLA-DRB104:05 | 458.8 | | HLA-DRB108:02 | 571.8 | | HLA-DRB301:01 | 196.5 | | VSARLLGDIVAVSKC | 521 | 535 | HLA-DPA102:01/DPB101:01 | 723.1 | | HLA-DPA103:01/DPB104:02 | 733.4 | | HLA-DQA101:02/DQB106:02 | 502.8 | | HLA-DQA105:01/DQB103:01 | 29.9 | | HLA-DRB103:01 | 214.3 | | HLA-DRB104:01 | 237 | | HLA-DRB104:05 | 429.4 | | HLA-DRB108:02 | 541.8 | | HLA-DRB301:01 | 172.3 | | SARLLGDIVAVSKCI | 522 | 536 | HLA-DPA102:01/DPB101:01 | 677.6 | | HLA-DPA103:01/DPB104:02 | 650.5 | | HLA-DQA101:02/DQB106:02 | 555.2 | | HLA-DQA105:01/DQB103:01 | 26.4 | | HLA-DRB101:01 | 175.3 | | HLA-DRB103:01 | 124.9 | | HLA-DRB104:01 | 187.9 | | HLA-DRB104:05 | 396.3 | | HLA-DRB108:02 | 396.7 | | HLA-DRB301:01 | 143.4 | | ARLLGDIVAVSKCIE | 523 | 537 | HLA-DPA102:01/DPB101:01 | 791.2 | | HLA-DPA103:01/DPB104:02 | 787.4 | | HLA-DQA101:02/DQB106:02 | 590.2 | | HLA-DQA105:01/DQB103:01 | 31.5 | | HLA-DRB101:01 | 270.9 | | HLA-DRB103:01 | 199.3 | | HLA-DRB104:01 | 255.3 | | HLA-DRB104:05 | 643.6 | | HLA-DRB108:02 | 360.5 | | HLA-DRB301:01 | 379.2 | | RLLGDIVAVSKCIEI | 524 | 538 | HLA-DPA102:01/DPB101:01 | 807.2 | | HLA-DPA103:01/DPB104:02 | 911.1 | | HLA-DQA101:02/DQB106:02 | 621.6 | | HLA-DQA105:01/DQB103:01 | 35.4 | | HLA-DRB103:01 | 347.3 | | HLA-DRB104:01 | 375.6 | | HLA-DRB104:05 | 977.9 | | HLA-DRB108:02 | 414.9 | | HLA-DRB301:01 | 974.2 | | LLGDIVAVSKCIEIP | 525 | 539 | HLA-DPA102:01/DPB101:01 | 941 | | HLA-DQA101:02/DQB106:02 | 748.6 | | HLA-DQA105:01/DQB103:01 | 37.1 | | HLA-DRB103:01 | 688 | | HLA-DRB104:01 | 848.5 | | HLA-DRB108:02 | 521 |
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Inhibitory concentration needed for binding MHC II to the IEDB NN-align method; the lower value is better. |
