Table 3: Stability analysis predicted by I-Mutant version 3.0 (also show the 23 novel mutations).

Amino Acid ChangeSVM2 Prediction EffectRIDDG Value Prediction

S749YDecrease0-0.2
F743SDecrease6-1.16
Y741CDecrease8-2.5
F731VDecrease6-1.52
I720TDecrease4-0.92
L709RDecrease3-0.56
V691GDecrease7-1.25
W689RDecrease7-0.73
G668RDecrease4-0.37
V659FDecrease2-0.19
F636CDecrease8-1.28
R461WIncrease1-0.01
H407QDecrease8-1.48
H407RDecrease5-1.1
H404RDecrease1-0.06
C398YDecrease2-0.09
C395YIncrease40.26
C395FIncrease10.04
C395RIncrease40.13
H378QDecrease4-0.68
H378YDecrease3-0.26
C375RIncrease 2 -0.01
L86PDecrease2-0.56