Detection and Estimation of alpha-Amyrin, beta-Sitosterol, Lupeol, and n-Triacontane in Two Medicinal Plants by High Performance Thin Layer Chromatography
Table 1
Method validation data for simultaneous quantification of alpha-amyrin, beta-sitosterol, lupeol, and n-triacontane from dried whole plant powder of Leptadenia reticulata (Retz.) Wight & Arn. and Pluchea lanceolata (DC.) CB. Clarke.
Parameters
Results
alpha-Amyrin
beta-Sitosterol
Lupeol
n-Triacontane
Linear range (µg/band)
0.1–1.0
0.1–1.0
0.1–1.0
1.0–10.0
Correlation coefficient ()
0.999
0.999
0.999
0.999
LOD (µg/band)
0.03
0.03
0.03
0.30
LOQ (µg/band)
0.10
0.10
0.10
1.00
Stability of standard solution
Stable for minimum 48 hours
Stable for minimum 48 hours
Stable for minimum 48 hours
Stable for minimum 48 hours
System suitability (% R.S.D.)
Less than 2
Less than 2
Less than 2
Less than 2
Leptadenia reticulata (Retz.) Wight & Arn.
Repeatability-% R.S.D. range () (on the same day)
Intermediate precision % R.S.D range () (percent R.S.D. for three successive days)
Assay (mg/2 g)
Percent recovery
Pluchea lanceolata (DC.) CB. Clarke.
Repeatability-% R.S.D. range () (on the same day)
N.D.
Intermediate precision % R.S.D range () (percent R.S.D. for three successive days)
