Development and Validation of Stability Indicating LC-PDA Method for Mycophenolate Mofetil in Presence of Mycophenolic Acid and Its Application for Degradation Kinetics and pH Profile Study

Table 1

Results of method optimization.

(a) Factors and their low (−), high (+), and zero (0) levels

Factors	Factors level
	(−)	(+)	(0)
Methanol % (𝑥1)	70	80	75
Flow rate (mL/min) (𝑥2)	0.60	0.70	0.65

(b) Obtained results for 𝑡_𝑅

Factors		Retention factors
𝑥1	𝑥2	MMF	MPA
−	−	8.64	7.67
−	+	6.44	5.68
+	−	7.69	6.46
+	+	5.70	4.79

(c) Effect of variables on 𝑡_𝑅 of MMF and MPA

Variable	% effect on 𝑡𝑅 of
	MMF	MMF
𝑥1 (% methanol)	13.94	38.51
𝑥2 (flow rate)	85.84	61.04
𝑥1𝑥2 (interaction)	0.22	0.45