Research Article

Inadvertent Transfer of Murine VL30 Retrotransposons to CAR-T Cells

Table 5

The table outlines the data employed in Figure 5(a). HIV-1 vector particles were generated in 293T cell clones 6 and 7 and employed on naive 293T cells. DNA was extracted from treated cells at either 24 h (P0) or following 4 passages (P4) after treatment and analyzed by qPCR for the VL30 genome copy number. The experiment was done in triplicate. The target cell line (293T cells) and the cell lines in which the HIV-1 particles containing conditioned media were generated are shown. The number of target cell passages (P0 and P4) is shown. Values of the VL30 genome copy number per target cell and the standard deviation (std) are shown.

Cell linePassageVL30 GCNStd

293T+P00.05830.0022
Clone 6 conditioned mediaP4Not detected
293T+P00.00780.0022
Clone 7 conditioned mediaP4Not detected