Research Article
Inadvertent Transfer of Murine VL30 Retrotransposons to CAR-T Cells
Table 6
Genome copy number (GCN) and vector copy number (VCN) of VL30 and -RVV (respectively), in DNA samples extracted from primary human T-cells as determined by qPCR analysis. DNA samples 1, 2, 5, 6, 7, 11, 12, and 13 were extracted from human T-cells treated with the -RVV SFG.iC9.GD2.CAR.IL15. DNA of samples 3, 4, 8, 9, and 10 extracted from naïve primary human T-cells and served as biological negative controls. DNA extracted from naïve 293T cells served as technical negative control (−cont). ND indicates that the level of targeted sequences (VL30 and -RVV) in the tested DNA sample was under the level of detection.
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