Research Article

Inadvertent Transfer of Murine VL30 Retrotransposons to CAR-T Cells

Table 8

Sequence homologies between various VL30 genomes and the human genome. The Genome Browser of the University of California Santa Cruz Genomic Institute (https://genome.ucsc.edu/) was employed to identify sequence homologies between various VL30 genomes and the human genome. The accession numbers associated with the sequence of the various VL30 genomes, which were employed in the VL30/human sequence homology screen, are indicated. The positions of the first and last (start and end, respectively) of VL30 nucleotides in the homologous sequence are shown. VL30 nucleotides that matched their respective nucleotides in homologous human sequences are shown in capital letters. The chromosome number of human chromosomes containing sequences with homology to VL30 genome sequences is indicated. The orientations of the relevant homologous strands are shown. The positions of the first and last (start and end, respectively) nucleotides in the human chromosomes with homology to the relevant VL30 sequence are shown. The repetitive sequences in the human chromosomes with homology to the abovementioned VL30 genomes are defined.

VL30Chromosome location

Accession numberStartEndVL30 sequenceChromosomeStrandStartEndRepeats
AF486451.136923844GATAATGGtCCTGCCTTTgttgCccaggTAagTCAGggtGTggccAAGtatTTagAggTcaAAtaaAAatTCCATTGTGtgTAcaGACCtCAGAGCTCaGaaaAGaTAaAAaAgAatAAatAaAaCtctAAAcAgAcctTgACAAAATTAATCChromosome 1+9003366990033696LINE
Chromosome 2+112924425113176587LINE
3886878738868812MER5A hAT-Charlie
+226901221226901241LINE
Chromosome 3+160777702160777732LINE
+1158035011580370LINE
Chromosome 4+167811047167811066LINE
Chromosome 6+128820061128820085LINE
Chromosome 7+3673167136731699LINE
Chromosome 14+3241778532417804
7680174276801771LINE
Chromosome 15+5404779654047817
M21123.113471375CTGTGTGtGTCT.TGTGTGTCTCTT.TGTGTChromosome 157447015744788SIMPLE
Chromosome 480276138027633
Chromosome 5117789902117790357SINE
+3816844438168464LINE
Chromosome 915926111592632
Chromosome 12+1204823512048332
Chromosome 18+4732531647325542LINE
+7982932679829346SIMPLE
Chromosome 194259516642595192SIMPLE
X17124.119381979GAAAAACGGTTTctGTgTgtgTcttTTtTgTCTCTTTGTGTTChromosome 4+121936835121936854LTR
Chromosome 67814838378148408SINE/LINE
Chromosome 10+4719194847191976SIMPLE
Chromosome 11+6632765966327696
16321676TGTCTgTATGTCTGTGTGTCTGTGTTGTGTGTCChromosome 1+117641953117642113LINE
Chromosome 1+3047742930477466SIMPLE
Chromosome 1+92940859294148SIMPLE
Chromosome 1+1510976015109794SIMPLE
Chromosome 1+113320956113320980SIMPLE
Chromosome 2+238126874238127155SIMPLE
Chromosome 2+8145968581459717SIMPLE
Chromosome 2120372884120372913SIMPLE
Chromosome 3+129370779129370806SIMPLE
Chromosome 4+136831470136831493SIMPLE
Chromosome 4+6551658165516600LTR
Chromosome 53834998138350374SIMPLE
Chromosome 689953748995414SIMPLE
Chromosome 6+166824634166824662SIMPLE
Chromosome 6161466706161466730SIMPLE
Chromosome 7+7372626973726328SIMPLE
Chromosome 8+141269742141269778SIMPLE
Chromosome 9136042391136042690LINE
Chromosome 9+138142919138142972
Chromosome 10+4322494743225088
Chromosome 10+38086513808959SIMPLE/LTR
Chromosome 11+131867607131867696
Chromosome 11+131681304131681336
Chromosome 1114915221491556
Chromosome 11+6906491869064941SIMPLE
Chromosome 12+9697685196976883SIMPLE
Chromosome 12+1525326615253296SIMPLE
Chromosome 1254815975481623SIMPLE
Chromosome 13109571450109571471SIMPLE
Chromosome 143859116538591197SIMPLE
Chromosome 149465119694651254SIMPLE
Chromosome 147442771974427757SIMPLE
Chromosome 17+2173773921737903SIMPLE
Chromosome 171546517415465214SIMPLE
Chromosome 188086281175
Chromosome 186319832963198833SIMPLE
Chromosome 20+6415389164153922SIMPLE
Chromosome 205343356153433587SIMPLE
Chromosome 212921375129214223
Chromosome X14165641416597SIMPLE
Chromosome Y14165641416597SIMPLE
AY940476.118991921ACAAAATTAATCCTAGAgACTGGChromosome 163527021535270237LTR
AY260554.191009133ATATTTGAATGTATttAGAAAAATAAacAAATAGChromosome 17125208571252108Gene ZRANB2 antisense/Arthur1 hAT-Tip100
Chromosome 10+1885924318859275LINE
Chromosome 10+2055376720553786

Bold capital is a matching base in VL30 sequences; the dot denoted a gap in the sequences.