Table of Contents
Advances in Ecology
Volume 2015, Article ID 394593, 6 pages
http://dx.doi.org/10.1155/2015/394593
Research Article

The Potential Spread of Mycobacterium tuberculosis into the Environment in the Creation of Spondylitis Tuberculosis Rabbit

1Orthopaedic and Traumatology Department, Faculty of Medicine, Cipto Mangunkusumo Hospital, University of Indonesia, Jakarta 10430, Indonesia
2Integrated Service Unit of Stem Cell Medical Technology, Cipto Mangunkusumo Hospital, Jakarta 10430, Indonesia
3Microbiology Department, Faculty of Medicine, University of Indonesia, Jakarta 10330, Indonesia
4Veterinary Teaching Hospital, Bogor Agricultural Institute, Bogor, West Java 16680, Indonesia

Received 3 July 2015; Revised 17 August 2015; Accepted 31 August 2015

Academic Editor: Alistair Bishop

Copyright © 2015 Ahmad Jabir Rahyussalim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Direct Mycobacterium tuberculosis inoculation on rabbit vertebral body was used in rabbit spinal infection study. The potential spread of Mycobacterium tuberculosis into the environment will be observed in order to create the conditions fulfilling biosafety aspects. Two groups of six New Zealand rabbits were treatment group () and control group (). The treatment group had injection of 0.1 mL (107 cfu/mL) suspension of Mycobacterium tuberculosis into the vertebral body T12. They were incubated for 2 to 14 weeks. One rabbit per period of 2, 4, 6, and 14 weeks was euthanized to collect feces, urine, saliva, and tissue lesions. The control group had only feces, urine, and saliva to detect bacteria using AFB staining, culture, and PCR. Both two groups were kept in individual cages. They were put together in a large cage for 3 hours every day to interact with each other. AFB staining, culture, and radiological examination showed negative result, but in one rabbit, histopathological examination showed positive result and PCR examination in another rabbit of the treatment group. Spreading score was 1.05% and infected score was 0 (null). The procedure did not reveal the potential spread of Mycobacterium tuberculosis into the environment.