Table of Contents
Advances in Endocrinology
Volume 2014, Article ID 830761, 6 pages
http://dx.doi.org/10.1155/2014/830761
Research Article

Lipid Peroxidation and Antioxidant Status in Nonobese Type 2 Diabetes Mellitus

1Department of Biochemistry, Institute of Post Graduate Medical Education and Research, Kolkata 700020, India
2Department of Biochemistry, ICARE Institute of Medical Sciences and Research, Banabishnupur, Haldia 721645, India

Received 30 May 2014; Revised 13 August 2014; Accepted 26 September 2014; Published 22 October 2014

Academic Editor: Sarantis Livadas

Copyright © 2014 Vineet Kumar Khemka et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of the study was to investigate the association between lipid peroxidation and antioxidant status in nonobese type 2 diabetes mellitus (T2DM) and further to correlate whether their significant association is putatively associated with the pathogenesis of T2DM. A number of 102 nonobese T2DM subjects and 95 nondiabetic subjects as healthy controls were enrolled in this cross-sectional study. Serum samples were collected in cryovials for malondialdehyde (MDA) and thiol assays. Total thiol or sulfhydryl (–SH) groups in peripheral blood mononuclear cells (PBMCs) and sera, as well as level of MDA, a marker for lipid peroxidation in serum, were measured spectrophotometrically. Serum MDA level was found significantly higher whereas serum and PBMC total thiol levels were diminished significantly among nonobese T2DM subjects compared to HC subjects. Moreover, serum MDA level is found to have a significant inverse correlation with serum total thiol and PBMC thiol levels among DM subjects, but no significant correlation was observed in HC individuals. A significant inverse correlation between serum MDA and serum total thiol levels among nonobese T2DM subjects suggests a close association of increased oxidative stress with decreased antioxidant status in nonobese T2DM.