Table of Contents
Advances in Endocrinology
Volume 2016 (2016), Article ID 3452760, 5 pages
Research Article

The Protective Effect of Testosterone on Indomethacin Induced Gastric Ulcer in Female Sprague Dawley Rats

1Department of Health Promotion and Education, Faculty of Public Health, University of Ibadan, Ibadan, Nigeria
2Department of Physiology, Faculty of Basic Medical sciences, College of Medicine, Ambrose Alli University, Ekpoma, Edo State, Nigeria

Received 2 August 2016; Revised 28 September 2016; Accepted 23 October 2016

Academic Editor: Oscar González-Flores

Copyright © 2016 U. Akpamu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Gastric ulcer has shown association with changes in sex hormones, with impact exacerbated in males. Also, males are known to be more exposed to ulcer risk factors. This study investigates the effect of testosterone on indomethacin induced gastric ulcers in adult female rats. Eighteen female rats (225 ± 25 g body weight) were randomly assigned to 3 groups under standard laboratory condition. After acclimatization, animals fasted for 40 hrs but were given water ad libitum. Group A served as control while group B served as the ulcer control, in which ulcer was induced without treatment using indomethacin (40 mg/kg single orally dose). Group C was pretreated with testosterone (1 mg/kg IM) eight hours before ulcer induction. Eight hours after ulcer induction, animals were sacrificed and the stomach was harvested for analysis. Results showed a significant reduction in mucus content in groups C ( g) and B ( g) compared to A ( g). Gastric mucus pH was significantly acidic in group B () compared to C () and A (). There was a significantly higher ulcer index in group B ( mm) compared to C ( mm) and testosterone pretreatment resulted in a 21.74% ulcer inhibition. Although weak, the findings suggest that testosterone might protect the gastric mucosa against NSAIDs in females.