Effect of Cycas pectinata Seed Extract on Testicular Steroidogenesis in a Mouse Model

Figure 7

Localization and expression of 17 βHSD in the testis after CPE treatment. The moderate immunostaining of 17 βHSD shown in the Leydig cells of 100 mg/kg (b) and 400 mg/kg (c) CPE-treated testis, whereas control showed mild immunostaining (a). The negative control section showed no immunostaining of 17 βHSD (d). The quantification of 17 βHSD-stained area showed a significant () increase in the staining area in the 100 mg/kg- and 400 mg/kg-treated groups compared to the control (e). The western blot analysis of 17 βHSD showed a significant () increased in the 400 mg/kg CPE-treated group compared to the control and 100 mg/kg-treated groups (g, f). Data are expressed as . Different alphabet letters above the bar graph indicate statistically significant differences between groups.

(a)

(b)

(c)

(d)

(e)

(f)

(g)