Curcumin Attenuates Ferroptosis and Ameliorates Erectile Function in Diabetic Rats by Activating Nrf2/HO-1 Pathway

Figure 7

Curcumin inhibits ferroptosis in penile endothelial cells via Nrf2/HO-1 signaling. CCECs were exposed to 60 mM glucose for 24 hr. After glucose treatment, CCECs were treated with DMSO or 30 μM curcumin for 24 hr. Representative stripes of western blot (a) and statistical analysis of Nrf2 and HO-1 (b). Realtime PCR analyses of Nrf2 and HO-1 (c) genes in CCECs. CCECs were stably transfected with Nrf2 siRNA and then exposed to 60 mM glucose for 24 hr. After glucose treatment, CCECs were treated with DMSO or 30 μM curcumin for 24 hr. CCK-8 assay for evaluating cell viability (d). Representative stripes of western blot (e), statistical analysis of MDA (f), xCT (g), and GPX4 (h) in CCECs. Data were presented as mean ± SD from three samples per group. compared with the NC group. ^# compared with the HG group. ^$ compared with the Nrf2 siRNA group. NC = black control; HG = CCECs treated with glucose; CUR = CCECs treated with glucose and curcumin; Nrf2 siRNA = CCECs were stably transfected with Nrf2 siRNA.

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