Table of Contents
Advances in Orthopedic Surgery
Volume 2014, Article ID 850594, 23 pages
http://dx.doi.org/10.1155/2014/850594
Review Article

Ultrastructure of Intervertebral Disc and Vertebra-Disc Junctions Zones as a Link in Etiopathogenesis of Idiopathic Scoliosis

1The Spine Center, Department of Orthopaedics, University of Colorado Denver, Aurora, CO 80045, USA
2Division of Basic Reproductive Sciences, Department of Obstetrics & Gynecology, University of Colorado Denver, Aurora, CO 80045, USA

Received 24 September 2013; Accepted 1 February 2014; Published 23 March 2014

Academic Editor: Chan S. Shim

Copyright © 2014 Evalina L. Burger et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. I. A. F. Stokes, R. G. Burwell, and P. H. Dangerfield, “Biomechanical spinal growth modulation and progressive adolescent scoliosis—a test of the 'vicious cycle' pathogenetic hypothesis: summary of an electronic focus group debate of the IBSE,” Scoliosis, vol. 1, no. 1, article 16, 2006. View at Publisher · View at Google Scholar · View at Scopus
  2. I. A. F. Stokes, H. Spence, D. D. Aronsson, and N. Kilmer, “Mechanical modulation of vertebral body growth: implications for scoliosis progression,” Spine, vol. 21, no. 10, pp. 1162–1167, 1996. View at Publisher · View at Google Scholar · View at Scopus
  3. S. Lupparelli, E. Pola, L. Pitta, O. Mazza, V. de Santis, and L. Aulisa, “Biomechanical factors affecting progression of structural scoliotic curves of the spine,” Studies in Health Technology and Informatics, vol. 91, pp. 81–85, 2002. View at Google Scholar · View at Scopus
  4. D. Fabris, S. Costantini, U. Nene, V. Lo Scalzo, and F. Finocchiaro, “The surgical treatment of adult lumbar scoliosis,” Chirurgia Narzadow Ruchu i Ortopedia Polska, vol. 69, no. 4, pp. 279–285, 2004. View at Google Scholar · View at Scopus
  5. J. F. Fraser, R. C. Huang, F. P. Girardi, and F. P. Cammisa Jr., “Pathogenesis, presentation, and treatment of lumbar spinal stenosis associated with coronal or sagittal spinal deformities,” Neurosurgical Focus, vol. 14, no. 1, article e6, 2003. View at Google Scholar · View at Scopus
  6. I. A. F. Stokes and L. Windisch, “Vertebral height growth predominates over intervertebral disc height growth in adolescents with scoliosis,” Spine, vol. 31, no. 14, pp. 1600–1604, 2006. View at Publisher · View at Google Scholar · View at Scopus
  7. C. T. Mehlman, A. Araghi, and D. R. Roy, “Hyphenated history: the Hueter-Volkmann law,” The American Journal of Orthopedics, vol. 26, no. 11, pp. 798–800, 1997. View at Google Scholar · View at Scopus
  8. S. L. Weinstein, The Pediatric Spine: Principles and Practice, Lippincott Williams & Wilkins, Philadelphia, Pa, USA, 2nd edition, 2001.
  9. I. A. Stokes, J. Gwadera, A. Dimock, C. E. Farnum, and D. D. Aronsson, “Modulation of vertebral and tibial growth by compression loading: diurnal versus full-time loading,” Journal of Orthopaedic Research, vol. 23, no. 1, pp. 188–195, 2005. View at Publisher · View at Google Scholar · View at Scopus
  10. M. R. Urban, J. C. T. Fairbank, S. R. S. Bibby, and J. P. G. Urban, “Intervertebral disc composition in neuromuscular scoliosis: changes in cell density and glycosaminoglycan concentration at the curve apex,” Spine, vol. 26, no. 6, pp. 610–617, 2001. View at Publisher · View at Google Scholar · View at Scopus
  11. R. G. Burwell, “Aetiology of idiopathic scoliosis: current concepts,” Pediatric Rehabilitation, vol. 6, no. 3-4, pp. 137–170, 2003. View at Publisher · View at Google Scholar · View at Scopus
  12. I. A. F. Stokes and D. D. Aronsson, “Disc and vertebral wedging in patients with progressive scoliosis,” Journal of Spinal Disorders, vol. 14, no. 4, pp. 317–322, 2001. View at Publisher · View at Google Scholar · View at Scopus
  13. P. Fernandes and S. L. Weinstein, “Natural history of early onset scoliosis,” Journal of Bone and Joint Surgery A, vol. 89, supplement 1, pp. 21–33, 2007. View at Publisher · View at Google Scholar · View at Scopus
  14. B. V. Reamy and J. B. Slakey, “Adolescent idiopathic scoliosis: review and current concepts,” The American Family Physician, vol. 64, no. 1, pp. 111–116, 2001. View at Google Scholar · View at Scopus
  15. J. W. Ogilvie, “Adult scoliosis: evaluation and nonsurgical treatment,” Instructional Course Lectures, vol. 41, pp. 251–255, 1992. View at Google Scholar · View at Scopus
  16. M. B. Dobbs and S. L. Weinstein, “Infantile and juvenile scoliosis,” Orthopedic Clinics of North America, vol. 30, no. 3, pp. 331–341, 1999. View at Publisher · View at Google Scholar · View at Scopus
  17. R. Wynne-Davies, “Familial (idiopathic) scoliosis. A family survey,” Journal of Bone and Joint Surgery B, vol. 50, no. 1, pp. 24–30, 1968. View at Google Scholar · View at Scopus
  18. O. Diedrich, A. von Strempel, M. Schloz, O. Schmitt, and C. N. Kraft, “Long-term observation and management of resolving infantile idiopathic scoliosis. A 25-year follow-up,” Journal of Bone and Joint Surgery B, vol. 84, no. 7, pp. 1030–1035, 2002. View at Google Scholar · View at Scopus
  19. S. L. Weinstein, D. C. Zavala, and I. V. Ponseti, “Idiopathic scoliosis. Long-term follow-up and prognosis in untreated patients,” Journal of Bone and Joint Surgery A, vol. 63, no. 5, pp. 702–712, 1981. View at Google Scholar · View at Scopus
  20. R. Wynne Davies, “Infantile idiopathic scoliosis. Causative factors, particularly in the first six months of life,” Journal of Bone and Joint Surgery B, vol. 57, no. 2, pp. 138–141, 1975. View at Google Scholar · View at Scopus
  21. P. Gupta, L. G. Lenke, and K. H. Bridwell, “Incidence of neural axis abnormalities in infantile and juvenile patients with spinal deformity: is a magnetic resonance image screening necessary?” Spine, vol. 23, no. 2, pp. 206–210, 1998. View at Publisher · View at Google Scholar · View at Scopus
  22. M. B. Dobbs, L. G. Lenke, D. A. Szymanski et al., “Prevelance of neural axis abnormalities in patients with infantile idiopathic scoliosis,” Journal of Bone and Joint Surgery A, vol. 84, no. 12, pp. 2230–2234, 2002. View at Google Scholar · View at Scopus
  23. U. M. Figueiredo and J. I. P. James, “Juvenile idiopathic scoliosis,” Journal of Bone and Joint Surgery B, vol. 63, no. 1, pp. 61–66, 1981. View at Google Scholar · View at Scopus
  24. V. T. Tolo and R. Gillespie, “The characteristics of juvenile idiopathic scoliosis and results of its treatment,” Journal of Bone and Joint Surgery B, vol. 60, no. 2, pp. 181–188, 1978. View at Google Scholar · View at Scopus
  25. Y. P. Charles, J.-P. Daures, V. de Rosa, and A. Diméglio, “Progression risk of idiopathic juvenile scoliosis during pubertal growth,” Spine, vol. 31, no. 17, pp. 1933–1942, 2006. View at Publisher · View at Google Scholar · View at Scopus
  26. W. J. Kane, “Scoliosis Prevalence: a call for a statement of terms,” Clinical Orthopaedics and Related Research, vol. 126, pp. 43–46, 1977. View at Google Scholar · View at Scopus
  27. T. B. Grivas, E. Vasiliadis, V. Mouzakis, C. Mihas, and G. Koufopoulos, “Association between adolescent idiopathic scoliosis prevalence and age at menarche in different geographic latitudes,” Scoliosis, vol. 1, no. 1, article 9, 2006. View at Publisher · View at Google Scholar · View at Scopus
  28. H.-K. Wong, J. H. P. Hui, U. Rajan, and H.-P. Chia, “Idiopathic scoliosis in Singapore schoolchildren: a prevalence study 15 years into the screening program,” Spine, vol. 30, no. 10, pp. 1188–1196, 2005. View at Publisher · View at Google Scholar · View at Scopus
  29. S.-W. Suh, H. N. Modi, J.-H. Yang, and J.-Y. Hong, “Idiopathic scoliosis in Korean schoolchildren: a prospective screening study of over 1 million children,” European Spine Journal, vol. 20, no. 7, pp. 1087–1094, 2011. View at Publisher · View at Google Scholar · View at Scopus
  30. L. Xu, X. Qiu, X. Sun et al., “Potential genetic markers predicting the outcome of brace treatment in patients with adolescent idiopathic scoliosis,” European Spine Journal, vol. 20, no. 10, pp. 1757–1764, 2011. View at Publisher · View at Google Scholar · View at Scopus
  31. H.-K. Wong and K.-J. Tan, “The natural history of adolescent idiopathic scoliosis,” Indian Journal of Orthopaedics, vol. 44, no. 1, pp. 9–13, 2010. View at Publisher · View at Google Scholar · View at Scopus
  32. P. N. Soucacos, K. Zacharis, K. Soultanis, J. Gelalis, T. Xenakis, and A. E. Beris, “Risk factors for idiopathic scoliosis: review of a 6-year prospective study,” Orthopedics, vol. 23, no. 8, pp. 833–838, 2000. View at Google Scholar · View at Scopus
  33. L.-E. Peterson, A. L. Nachemson, D. S. Bradford et al., “Prediction of progression of the curve in girls who have adolescent idiopathic scoliosis of moderate severity. Logistic regression analysis based on data from the Brace Study of the Scoliosis Research Society,” Journal of Bone and Joint Surgery A, vol. 77, no. 6, pp. 823–827, 1995. View at Google Scholar · View at Scopus
  34. J. R. Davids, E. Chamberlin, and D. W. Blackhurst, “Indications for magnetic resonance imaging in presumed adolescent idiopathic scoliosis,” Journal of Bone and Joint Surgery A, vol. 86, no. 10, pp. 2187–2195, 2004. View at Google Scholar · View at Scopus
  35. M. O. Andersen, K. Thomsen, and K. O. Kyvik, “Adolescent idiopathic scoliosis in twins: a population-based survey,” Spine, vol. 32, no. 8, pp. 927–930, 2007. View at Publisher · View at Google Scholar · View at Scopus
  36. R. G. Burwell, A. A. Cole, T. A. Cook et al., “Pathogenesis of idiopathic scoliosis. The Nottingham concept,” Acta Orthopaedica Belgica, vol. 58, supplement 1, pp. 33–58, 1992. View at Google Scholar · View at Scopus
  37. R. G. Burwell, B. J. Freeman, P. H. Dangerfield et al., “Etiologic theories of idiopathic scoliosis: neurodevelopmental concept of maturational delay of the CNS body schema (“body-in-the-brain”),” Studies in Health Technology and Informatics, vol. 123, pp. 72–79, 2006. View at Google Scholar · View at Scopus
  38. J. A. Sevastik, “Dysfunction of the autonomic nerve system (ANS) in the aetiopathogenesis of adolescent idiopathic scoliosis,” Studies in Health Technology and Informatics, vol. 88, pp. 20–23, 2002. View at Google Scholar · View at Scopus
  39. C. Barrios and J. I. Arrotegui, “Experimental kyphoscoliosis induced in rats by selective brain stem damage,” International Orthopaedics, vol. 16, no. 2, pp. 146–151, 1992. View at Google Scholar · View at Scopus
  40. R. Herman, J. Mixon, and A. Fisher, “Idiopathic scoliosis and the central nervous system: a motor control problem. The Harrington Lecture, 1983. Scoliosis Research Society,” Spine, vol. 10, no. 1, pp. 1–14, 1985. View at Google Scholar · View at Scopus
  41. X. Sun, Y. Qiu, and Z. Zhu, “Variations of the position of the cerebellar tonsil in adolescent idiopathic scoliosis with severe curves: a MRI study,” Studies in Health Technology and Informatics, vol. 123, pp. 565–570, 2006. View at Google Scholar · View at Scopus
  42. Í. T. Benli, O. Üzümcügil, E. Aydin, B. Ateş, L. Gürses, and B. Hekimoǧlu, “Magnetic resonance imaging abnormalities of neural axis in Lenke type 1 idiopathic scoliosis,” Spine, vol. 31, no. 16, pp. 1828–1833, 2006. View at Publisher · View at Google Scholar · View at Scopus
  43. A. E. Oestreich, L. W. Young, and T. Y. Poussaint, “Scoliosis circa 2000: radiologic imaging perspective. I. Diagnosis and pretreatment evaluation,” Skeletal Radiology, vol. 27, no. 11, pp. 591–605, 1998. View at Publisher · View at Google Scholar · View at Scopus
  44. X. Guo, W. W. Chau, C. W. Y. Hui-Chan, C. S. K. Cheung, W. W. N. Tsang, and J. C. Y. Cheng, “Balance control in adolescents with idiopathic scoliosis and disturbed somatosensory function,” Spine, vol. 31, no. 14, pp. E437–E440, 2006. View at Publisher · View at Google Scholar · View at Scopus
  45. M. L. M. Lao, D. H. K. Chow, X. Guo, J. C. Y. Cheng, and A. D. Holmes, “Impaired dynamic balance control in adolescents with idiopathic scoliosis and abnormal somatosensory evoked potentials,” Journal of Pediatric Orthopaedics, vol. 28, no. 8, pp. 846–849, 2008. View at Publisher · View at Google Scholar · View at Scopus
  46. M. Beaulieu, C. Toulotte, L. Gatto et al., “Postural imbalance in non-treated adolescent idiopathic scoliosis at different periods of progression,” European Spine Journal, vol. 18, no. 1, pp. 38–44, 2009. View at Publisher · View at Google Scholar · View at Scopus
  47. R. G. Burwell, P. H. Dangerfield, B. J. Freeman et al., “Etiologic theories of idiopathic scoliosis: the breaking of bilateral symmetry in relation to left-right asymmetry of internal organs, right thoracic adolescent idiopathic scoliosis (AIS) and vertebrate evolution,” Studies in Health Technology and Informatics, vol. 123, pp. 385–390, 2006. View at Google Scholar · View at Scopus
  48. C. J. Goldberg, F. E. Dowling, E. E. Fogarty, and D. P. Moore, “Adolescent idiopathic scoliosis as developmental instability,” Genetica, vol. 96, no. 3, pp. 247–255, 1995. View at Publisher · View at Google Scholar · View at Scopus
  49. C. J. Goldberg, E. E. Fogarty, D. P. Moore, and F. E. Dowling, “Scoliosis and developmental theory: adolescent idiopathic scoliosis,” Spine, vol. 22, no. 19, pp. 2228–2238, 1997. View at Publisher · View at Google Scholar · View at Scopus
  50. R. G. Burwell, N. J. James, and F. Johnson, “Standardised trunk asymmetry scores. A study of back contour in healthy schoolchildren,” Journal of Bone and Joint Surgery B, vol. 65, no. 4, pp. 452–463, 1983. View at Google Scholar · View at Scopus
  51. A. E. Geissele, M. J. Kransdorf, C. A. Geyer, J. S. Jelinek, and B. E. van Dam, “Magnetic resonance imaging of the brain stem in adolescent idiopathic scoliosis,” Spine, vol. 16, no. 7, pp. 761–763, 1991. View at Google Scholar · View at Scopus
  52. M. Nissinen, M. Heliovaara, J. Seitsamo, and M. Poussa, “Trunk asymmetry, posture, growth, and risk of scoliosis: a three-year follow-up of Finnish prepubertal school children,” Spine, vol. 18, no. 1, pp. 8–13, 1993. View at Publisher · View at Google Scholar · View at Scopus
  53. H. Normelli, J. A. Sevastik, G. Ljung, and A.-M. Jonsson-Soderstrom, “The symmetry of the breasts in normal and scoliotic girls,” Spine, vol. 11, no. 7, pp. 749–752, 1986. View at Google Scholar · View at Scopus
  54. M. Pecina, O. Lulic-Dukic, and A. Pecina-Hrncevic, “Hereditary orthodontic anomalies and idiopathic scoliosis,” International Orthopaedics, vol. 15, no. 1, pp. 57–59, 1991. View at Google Scholar · View at Scopus
  55. M. J. Saji, S. S. Upadhyay, and J. C. Y. Leong, “Increased femoral neck-shaft angles in adolescent idiopathic scoliosis,” Spine, vol. 20, no. 3, pp. 303–311, 1995. View at Google Scholar · View at Scopus
  56. M. P. Wyatt, R. L. Barrack, and S. J. Mubarak, “Vibratory response in idiopathic scoliosis,” Journal of Bone and Joint Surgery B, vol. 68, no. 5, pp. 714–718, 1986. View at Google Scholar · View at Scopus
  57. M. Machida, J. Dubousset, Y. Imamura, T. Iwaya, T. Yamada, and J. Kimura, “An experimental study in chickens for the pathogenesis of idiopathic scoliosis,” Spine, vol. 18, no. 12, pp. 1609–1615, 1993. View at Google Scholar · View at Scopus
  58. M. Machida, J. Dubousset, Y. Imamura, Y. Iwaya, T. Yamada, and J. Kimura, “Role of melatonin deficiency in the development of scoliosis in pinealectomised chickens,” Journal of Bone and Joint Surgery B, vol. 77, no. 1, pp. 134–138, 1995. View at Google Scholar · View at Scopus
  59. J. Dubousset and M. Machida, “Possible role of pineal gland in pathogenesis of idiopathic scoliosis. Experimental and clinical studies,” Bulletin de l'Academie Nationale de Medecine, vol. 185, no. 3, pp. 593–604, 2001. View at Google Scholar · View at Scopus
  60. M. Machida, J. Dubousset, T. Yamada et al., “Experimental scoliosis in melatonin-deficient C57BL/6J mice without pinealectomy,” Journal of Pineal Research, vol. 41, no. 1, pp. 1–7, 2006. View at Publisher · View at Google Scholar · View at Scopus
  61. M. Machida, J. Dubousset, Y. Imamura, Y. Miyashita, T. Yamada, and J. Kimura, “Melatonin: a possible role in pathogenesis of adolescent idiopathic scoliosis,” Spine, vol. 21, no. 10, pp. 1147–1152, 1996. View at Publisher · View at Google Scholar · View at Scopus
  62. K. M. C. Cheung, T. Wang, A. M. S. Poon et al., “The effect of pinealectomy on scoliosis development in young nonhuman primates,” Spine, vol. 30, no. 18, pp. 2009–2013, 2005. View at Publisher · View at Google Scholar · View at Scopus
  63. K. M. Bagnall, V. J. Raso, D. L. Hill et al., “Melatonin levels in idiopathic scoliosis: diurnal and nocturnal serum melatonin levels in girls with adolescent idiopathic scoliosis,” Spine, vol. 21, no. 17, pp. 1974–1978, 1996. View at Publisher · View at Google Scholar · View at Scopus
  64. A. S. Hilibrand, L. C. Blakemore, R. T. Loder et al., “The role of melatonin in the pathogenesis of adolescent idiopathic scoliosis,” Spine, vol. 21, no. 10, pp. 1140–1146, 1996. View at Publisher · View at Google Scholar · View at Scopus
  65. A. B. Fagan, D. J. Kennaway, and A. D. Sutherland, “Total 24-hour melatonin secretion in adolescent idiopathic scoliosis: a case-control study,” Spine, vol. 23, no. 1, pp. 41–46, 1998. View at Publisher · View at Google Scholar · View at Scopus
  66. W. Brodner, P. Krepler, M. Nicolakis et al., “Melatonin and adolescent idiopathic scoliosis,” Journal of Bone and Joint Surgery B, vol. 82, no. 3, pp. 399–403, 2000. View at Google Scholar · View at Scopus
  67. K. T. Suh, S. S. Lee, S. J. Kim, Y. K. Kim, and J. S. Lee, “Pineal gland metabolism in patients with adolescent idiopathic scoliosis,” Journal of Bone and Joint Surgery B, vol. 89, no. 1, pp. 66–71, 2007. View at Publisher · View at Google Scholar · View at Scopus
  68. A. Moreau, D. S. Wang, S. Forget et al., “Melatonin signaling dysfunction in adolescent idiopathic scoliosis,” Spine, vol. 29, no. 16, pp. 1772–1781, 2004. View at Publisher · View at Google Scholar · View at Scopus
  69. B. Azeddine, K. Letellier, D. S. Wang, F. Moldovan, and A. Moreau, “Molecular determinants of melatonin signaling dysfunction in adolescent idiopathic scoliosis,” Clinical Orthopaedics and Related Research, no. 462, pp. 45–52, 2007. View at Publisher · View at Google Scholar · View at Scopus
  70. L. H. S. Sekhon, N. Duggal, J. J. Lynch et al., “Magnetic resonance imaging clarity of the Bryan, Prodisc-C, Prestige LP, and PCM cervical arthroplasty devices,” Spine, vol. 32, no. 6, pp. 673–680, 2007. View at Publisher · View at Google Scholar · View at Scopus
  71. X. S. Qiu, N. L. S. Tang, H. Y. Yeung et al., “Melatonin receptor 1B (MTNR1B) gene polymorphism is associated with the occurrence of adolescent idiopathic scoliosis,” Spine, vol. 32, no. 16, pp. 1748–1753, 2007. View at Publisher · View at Google Scholar · View at Scopus
  72. J. A. Morcuende, R. Minhas, L. Dolan et al., “Allelic variants of human melatonin 1A receptor in patients with familial adolescent idiopathic scoliosis,” Spine, vol. 28, no. 17, pp. 2025–2028, 2003. View at Publisher · View at Google Scholar · View at Scopus
  73. M. I. Masana, J. M. Soares Jr., and M. L. Dubocovich, “17β-Estradiol modulates hMT1 melatonin receptor function,” Neuroendocrinology, vol. 81, no. 2, pp. 87–95, 2005. View at Publisher · View at Google Scholar · View at Scopus
  74. K. Letellier, B. Azeddine, S. Parent et al., “Estrogen cross-talk with the melatonin signaling pathway in human osteoblasts derived from adolescent idiopathic scoliosis patients,” Journal of Pineal Research, vol. 45, no. 4, pp. 383–393, 2008. View at Publisher · View at Google Scholar · View at Scopus
  75. J. Vanecek, “Cellular mechanisms of melatonin action,” Physiological Reviews, vol. 78, no. 3, pp. 687–721, 1998. View at Google Scholar · View at Scopus
  76. P. Das, D. J. Schurman, and R. L. Smith, “Nitric oxide and G proteins mediate the response of bovine articular chondrocytes to fluid-induced shear,” Journal of Orthopaedic Research, vol. 15, no. 1, pp. 87–93, 1997. View at Publisher · View at Google Scholar · View at Scopus
  77. A. J. El Haj, L. M. Walker, M. R. Preston, and S. J. Publicover, “Mechanotransduction pathways in bone: calcium fluxes and the role of voltage-operated calcium channels,” Medical and Biological Engineering and Computing, vol. 37, no. 3, pp. 403–409, 1999. View at Google Scholar · View at Scopus
  78. G. R. Erickson, L. G. Alexopoulos, and F. Guilak, “Hyper-osmotic stress induces volume change and calcium transients in chondrocytes by transmembrane, phospholipid, and G-protein pathways,” Journal of Biomechanics, vol. 34, no. 12, pp. 1527–1535, 2001. View at Publisher · View at Google Scholar · View at Scopus
  79. F. M. Pavalko, S. M. Norvell, D. B. Burr, C. H. Turner, R. L. Duncan, and J. P. Bidwell, “A model for mechanotransduction in bone cells: the load-bearing mechanosomes,” Journal of Cellular Biochemistry, vol. 88, no. 1, pp. 104–112, 2003. View at Publisher · View at Google Scholar · View at Scopus
  80. A. Conti, S. Conconi, E. Hertens, K. Skwarlo-Sonta, M. Markowska, and G. J. M. Maestroni, “Evidence for melatonin synthesis in mouse and human bone marrow cells,” Journal of Pineal Research, vol. 28, no. 4, pp. 193–202, 2000. View at Google Scholar · View at Scopus
  81. J. A. Roth, B.-G. Kim, F. Song, W.-L. Lin, and M.-I. Cho, “Melatonin promotes osteoblast differentiation and bone formation,” Journal of Biological Chemistry, vol. 274, no. 31, pp. 22041–22047, 1999. View at Google Scholar · View at Scopus
  82. H. Koyama, O. Nakade, Y. Takada, T. Kaku, and K.-H. W. Lau, “Melatonin at pharmacologic doses increases bone mass by suppressing resorption through down-regulation of the RANKL-mediated osteoclast formation and activation,” Journal of Bone and Mineral Research, vol. 17, no. 7, pp. 1219–1229, 2002. View at Google Scholar · View at Scopus
  83. N. Suzuki and A. Hattori, “Melatonin suppresses osteoclastic and osteoblastic activities in the scales of goldfish,” Journal of Pineal Research, vol. 33, no. 4, pp. 253–258, 2002. View at Publisher · View at Google Scholar · View at Scopus
  84. D. P. Cardinali, M. G. Ladizesky, V. Boggio, R. A. Cutrera, and C. Mautalen, “Melatonin effects on bone: experimental facts and clinical perspectives,” Journal of Pineal Research, vol. 34, no. 2, pp. 81–87, 2003. View at Publisher · View at Google Scholar · View at Scopus
  85. M. Ylikoski, “Growth and progression of adolescent idiopathic scoliosis in girls,” Journal of Pediatric Orthopaedics Part B, vol. 14, no. 5, pp. 320–324, 2005. View at Google Scholar · View at Scopus
  86. X. Guo, W.-W. Chau, Y.-L. Chan, and J. C.-Y. Cheng, “Relative anterior spinal overgrowth in adolescent idiopathic scoliosis,” Journal of Bone and Joint Surgery B, vol. 85, no. 7, pp. 1026–1031, 2003. View at Publisher · View at Google Scholar · View at Scopus
  87. X. Guo, W.-W. Chau, Y.-L. Chan, J.-C.-Y. Cheng, R. G. Burwell, and P. H. Dangerfield, “Relative anterior spinal overgrowth in adolescent idiopathic scoliosis—result of disproportionate endochondral-membranous bone growth? Summary of an electronic focus group debate of the IBSE,” European Spine Journal, vol. 14, no. 9, pp. 862–873, 2005. View at Publisher · View at Google Scholar · View at Scopus
  88. R. W. Porter, “The pathogenesis of idiopathic scoliosis: uncoupled neuro-osseous growth?” European Spine Journal, vol. 10, no. 6, pp. 473–481, 2001. View at Publisher · View at Google Scholar · View at Scopus
  89. R. Yarom and G. C. Robin, “Studies on spinal and peripheral muscles from patients with scoliosis,” Spine, vol. 4, no. 1, pp. 12–21, 1979. View at Google Scholar · View at Scopus
  90. R. Yarom, A. Muhlrad, S. Hodges, and G. C. Robin, “Platelet pathology in patients with idiopathic scoliosis. Ultrastructural morphometry, aggregations, X-ray spectrometry, and biochemical analysis,” Laboratory Investigation, vol. 43, no. 3, pp. 208–216, 1980. View at Google Scholar · View at Scopus
  91. T. G. Lowe, M. Edgar, J. Y. Margulies et al., “Etiology of idiopathic scoliosis: current trends in research,” Journal of Bone and Joint Surgery A, vol. 82, no. 8, pp. 1157–1168, 2000. View at Google Scholar · View at Scopus
  92. K. Kindsfater, T. Lowe, D. Lawellin, D. Weinstein, and J. Akmakjian, “Levels of platelet calmodulin for the prediction of progression and severity of adolescent idiopathic scoliosis,” Journal of Bone and Joint Surgery A, vol. 76, no. 8, pp. 1186–1192, 1994. View at Google Scholar · View at Scopus
  93. T. Lowe, D. Lawellin, D. Smith et al., “Platelet calmodulin levels in adolescent idiopathic scoliosis: do the levels correlate with curve progression and severity?” Spine, vol. 27, no. 7, pp. 768–775, 2002. View at Publisher · View at Google Scholar · View at Scopus
  94. T. G. Lowe, R. G. Burwell, and P. H. Dangerfield, “Platelet calmodulin levels in adolescent idiopathic scoliosis (AIS): can they predict curve progression and severity? Summary of an electronic focus group debate of the IBSE,” European Spine Journal, vol. 13, no. 3, pp. 257–265, 2004. View at Publisher · View at Google Scholar · View at Scopus
  95. R. G. Burwell and P. H. Dangerfield, “Pathogenesis of progressive adolescent idiopathic scoliosis platelet activation and vascular biology in immature vertebrae: an alternative molecular hypothesis,” Acta Orthopaedica Belgica, vol. 72, no. 3, pp. 247–260, 2006. View at Google Scholar · View at Scopus
  96. M. I. Vacas, M. M. de las del Zar, M. Martinuzzo, and D. P. Cardinali, “Binding sites for [3H]-melatonin in human platelets,” Journal of Pineal Research, vol. 13, no. 2, pp. 60–65, 1992. View at Publisher · View at Google Scholar · View at Scopus
  97. D. P. Cardinali, M. M. del Zar, and M. I. Vacas, “The effects of melatonin in human platelets,” Acta Physiologica Pharmacologica et Therapeutica Latinoamericana, vol. 43, no. 1-2, pp. 1–13, 1993. View at Google Scholar · View at Scopus
  98. D. P. Cardinali, D. A. Golombek, R. E. Rosenstein, R. A. Cutrera, and A. I. Esquifino, “Melatonin site and mechanism of action: single or multiple?” Journal of Pineal Research, vol. 23, no. 1, pp. 32–39, 1997. View at Google Scholar · View at Scopus
  99. J. Yang, J. Wu, M. Anna Kowalska et al., “Loss of signaling through the G protein, G(z), results in abnormal platelet activation and altered responses to psychoactive drugs,” Proceedings of the National Academy of Sciences of the United States of America, vol. 97, no. 18, pp. 9984–9989, 2000. View at Google Scholar · View at Scopus
  100. S. I. S. Se Il Suk, I. K. K. In Kwon Kim, C. K. L. Choon Ki Lee, Y. D. K. Young Do Koh, and J. S. Y. Jin Sup Yeom, “A study on platelet function in idiopathic scoliosis,” Orthopedics, vol. 14, no. 10, pp. 1079–1083, 1991. View at Google Scholar · View at Scopus
  101. W. K. Ho, M. Baccala, J. Thom, and J. W. Eikelboom, “High prevalence of abnormal preoperative coagulation tests in patients with adolescent idiopathic scoliosis,” Journal of Thrombosis and Haemostasis, vol. 3, no. 5, pp. 1094–1095, 2005. View at Publisher · View at Google Scholar · View at Scopus
  102. K. Freson, V. Labarque, C. Thys, C. Wittevrongel, and C. V. Geet, “What's new in using platelet research? To unravel thrombopathies and other human disorders,” European Journal of Pediatrics, vol. 166, no. 12, pp. 1203–1210, 2007. View at Publisher · View at Google Scholar · View at Scopus
  103. A. Pletscher, “Blood platelets as neuronal models: use and limitations,” Clinical Neuropharmacology, vol. 9, pp. 344–346, 1986. View at Google Scholar · View at Scopus
  104. N. Hadley-Miller, B. Mims, and D. M. Milewicz, “The potential role of the elastic fiber system in adolescent idiopathic scoliosis,” Journal of Bone and Joint Surgery A, vol. 76, no. 8, pp. 1193–1206, 1994. View at Google Scholar · View at Scopus
  105. G. M. Corson, N. L. Charbonneau, D. R. Keene, and L. Y. Sakai, “Differential expression of fibrillin-3 adds to microfibril variety in human and avian, but not rodent, connective tissues,” Genomics, vol. 83, no. 3, pp. 461–472, 2004. View at Publisher · View at Google Scholar · View at Scopus
  106. E. G. Cleary and M. A. Gibson, “Elastin-associated microfibrils and microfibrillar proteins,” International Review of Connective Tissue Research, vol. 10, pp. 97–209, 1983. View at Google Scholar · View at Scopus
  107. M. K. Chelberg, G. M. Banks, D. F. Geiger, and T. R. Oegema Jr., “Identification of heterogeneous cell populations in normal human intervertebral disc,” Journal of Anatomy, vol. 186, part 1, pp. 43–53, 1995. View at Google Scholar · View at Scopus
  108. M. Turgut, G. Öktem, A. Uysal, and M. E. Yurtseven, “Immunohistochemical profile of transforming growth factor-β1 and basic fibroblast growth factor in sciatic nerve anastomosis following pinealectomy and exogenous melatonin administration in rats,” Journal of Clinical Neuroscience, vol. 13, no. 7, pp. 753–758, 2006. View at Publisher · View at Google Scholar · View at Scopus
  109. L. B. Skogland and J. A. A. Miller, “The length and proportions of the thoracolumbar spine in children with idiopathic scoliosis,” Acta Orthopaedica Scandinavica, vol. 52, no. 2, pp. 177–185, 1981. View at Google Scholar · View at Scopus
  110. L. B. Skogland and J. A. A. Miller, “Growth related hormones in idiopathic scoliosis. An endocrine basis for accelerated growth,” Acta Orthopaedica Scandinavica, vol. 51, no. 5, pp. 779–789, 1980. View at Google Scholar · View at Scopus
  111. T. Ahl, K. Albertsson-Wikland, and R. Kalen, “Twenty-four-hour growth hormone profiles in pubertal girls with idiopathic scoliosis,” Spine, vol. 13, no. 2, pp. 139–142, 1988. View at Google Scholar · View at Scopus
  112. L. B. Skogland, J. A. A. Miller, A. Skottner, and L. Fryklund, “Serum somatomedin A and non-dialyzable urinary hydroxyproline in girls with idiopathic scoliosis,” Acta Orthopaedica Scandinavica, vol. 52, no. 3, pp. 307–313, 1981. View at Google Scholar · View at Scopus
  113. E. D. Wang, D. S. Drummond, J. P. Dormans, T. Moshang, R. S. Davidson, and D. Gruccio, “Scoliosis in patients treated with growth hormone,” Journal of Pediatric Orthopaedics, vol. 17, no. 6, pp. 708–711, 1997. View at Publisher · View at Google Scholar · View at Scopus
  114. J. F. Dymling and S. Willner, “Progression of a structural scoliosis during treatment with growth hormone. A case report,” Acta Orthopaedica Scandinavica, vol. 49, no. 3, pp. 264–268, 1978. View at Google Scholar · View at Scopus
  115. G. A. Day, I. B. McPhee, J. Batch, and F. H. Tomlinson, “Growth rates and the prevalence and progression of scoliosis in short-statured children on Australian growth hormone treatment programmes,” Scoliosis, vol. 2, no. 1, article 3, 2007. View at Publisher · View at Google Scholar · View at Scopus
  116. C. J. Rosen and L. R. Donahue, “Insulin-like growth factors and bone: the osteoporosis connection revisited,” Proceedings of the Society for Experimental Biology and Medicine, vol. 219, no. 1, pp. 1–7, 1998. View at Google Scholar · View at Scopus
  117. X. S. Qiu, N. L. S. Tang, H.-Y. Yeung, Y. Qiu, and J. C. Y. Cheng, “Genetic association study of growth hormone receptor and idiopathic scoliosis,” Clinical Orthopaedics and Related Research, vol. 462, pp. 53–58, 2007. View at Publisher · View at Google Scholar · View at Scopus
  118. Y. Yang, Z. Wu, T. Zhao et al., “Adolescent idiopathic scoliosis and the single-nucleotide polymorphism of the growth hormone receptor and IGF-1 genes,” Orthopedics, vol. 32, no. 6, article 411, 2009. View at Publisher · View at Google Scholar · View at Scopus
  119. E. S. Moon, H. S. Kim, V. Sharma et al., “Analysis of single nucleotide polymorphism in adolescent idiopathic scoliosis in Korea: for personalized treatment,” Yonsei Medical Journal, vol. 54, no. 2, pp. 500–509, 2013. View at Publisher · View at Google Scholar
  120. J. Falcón, L. Besseau, D. Fazzari et al., “Melatonin modulates secretion of growth hormone and prolactin by trout pituitary glands and cells in culture,” Endocrinology, vol. 144, no. 10, pp. 4648–4658, 2003. View at Publisher · View at Google Scholar · View at Scopus
  121. Z. Ostrowska, B. Kos-Kudla, E. Swietochowska, B. Marek, D. Kajdaniuk, and N. Ciesielska-Kopacz, “Influence of pinealectomy and long-term melatonin administration on GH-IGF-I axis function in male rats,” Neuroendocrinology Letters, vol. 22, no. 4, pp. 255–262, 2001. View at Google Scholar · View at Scopus
  122. P. Lissoni, M. Cazzaniga, G. Tancini et al., “Reversal of clinical resistance to LHRH analogue in metastatic prostate cancer by the pineal hormone melatonin: efficacy of LHRH analogue plus melatonin in patients progressing on LHRH analogue alone,” European Urology, vol. 31, no. 2, pp. 178–181, 1997. View at Google Scholar · View at Scopus
  123. J. O. Sanders, R. H. Browne, S. J. McConnell, S. A. Margraf, T. E. Cooney, and D. N. Finegold, “Maturity assessment and curve progression in girls with idiopathic scoliosis,” Journal of Bone and Joint Surgery A, vol. 89, no. 1, pp. 64–73, 2007. View at Publisher · View at Google Scholar · View at Scopus
  124. J. W. Raczkowski, “The concentrations of testosterone and estradiol in girls with adolescent idiopathic scoliosis,” Neuroendocrinology Letters, vol. 28, no. 3, pp. 302–304, 2007. View at Google Scholar · View at Scopus
  125. T. Esposito, R. Uccello, R. Caliendo et al., “Estrogen receptor polymorphism, estrogen content and idiopathic scoliosis in human: a possible genetic linkage,” Journal of Steroid Biochemistry and Molecular Biology, vol. 116, no. 1-2, pp. 56–60, 2009. View at Publisher · View at Google Scholar · View at Scopus
  126. K. Venken, S. Movérare-Skrtic, J. J. Kopchick et al., “Impact of androgens, growth hormone, and IGF-I on bone and muscle in male mice during puberty,” Journal of Bone and Mineral Research, vol. 22, no. 1, pp. 72–82, 2007. View at Publisher · View at Google Scholar · View at Scopus
  127. P. Raz, E. Nasatzky, B. D. Boyan, A. Ornoy, and Z. Schwartz, “Sexual dimorphism of growth plate prehypertrophic and hypertrophic chondrocytes in response to testosterone requires metabolism to dihydrotestosterone (DHT) by steroid 5-alpha reductase type 1,” Journal of Cellular Biochemistry, vol. 95, no. 1, pp. 108–119, 2005. View at Publisher · View at Google Scholar · View at Scopus
  128. R. Luboshitzky, O. Wagner, S. Lavi, P. Herer, and P. Lavie, “Abnormal melatonin secretion in hypogonadal men: the effect of testosterone treatment,” Clinical Endocrinology, vol. 47, no. 4, pp. 463–469, 1997. View at Google Scholar · View at Scopus
  129. A. Kulis, D. Zarzycki, and J. Jaśkiewicz, “Concentration of estradiol in girls with idiophatic scoliosis,” Ortopedia Traumatologia Rehabilitacja, vol. 8, no. 4, pp. 455–459, 2006. View at Google Scholar · View at Scopus
  130. A. Kulis and J. Jaśkiewicz, “Concentration of selected regulators of calciumphosphate balance in girls with idiopathic scoliosis,” Ortopedia Traumatologia Rehabilitacja, vol. 11, no. 5, pp. 438–447, 2009. View at Google Scholar · View at Scopus
  131. C. J. Goldberg, F. E. Dowling, and E. E. Fogarty, “Adolescent idiopathic scoliosis—early menarche, normal growth,” Spine, vol. 18, no. 5, pp. 529–535, 1993. View at Google Scholar · View at Scopus
  132. H. Normelli, J. Sevastik, and G. Ljung, “Anthropometric data relating to normal and scoliotic Scandinavian girls,” Spine, vol. 10, no. 2, pp. 123–126, 1985. View at Google Scholar · View at Scopus
  133. W. T. K. Lee, C. S. K. Cheung, Y. K. Tse et al., “Association of osteopenia with curve severity in adolescent idiopathic scoliosis: a study of 919 girls,” Osteoporosis International, vol. 16, no. 12, pp. 1924–1932, 2005. View at Publisher · View at Google Scholar · View at Scopus
  134. J. C. Cheng, S. P. Tang, X. Guo, C. W. Chan, and L. Qin, “Osteopenia in adolescent idiopathic scoliosis: a histomorphometric study,” Spine, vol. 26, no. 3, pp. E19–E23, 2001. View at Google Scholar · View at Scopus
  135. M. N. Weitzmann and R. Pacifici, “Estrogen regulation of immune cell bone interactions,” Annals of the New York Academy of Sciences, vol. 1068, no. 1, pp. 256–274, 2006. View at Publisher · View at Google Scholar · View at Scopus
  136. A. Bouhoute and G. Leclercq, “Calmodulin decreases the estrogen binding capacity of the estrogen receptor,” Biochemical and Biophysical Research Communications, vol. 227, no. 3, pp. 651–657, 1996. View at Publisher · View at Google Scholar · View at Scopus
  137. A. S. Dusso and A. J. Brown, “Mechanism of vitamin D action and its regulation,” The American Journal of Kidney Diseases, vol. 32, supplement 2, pp. S13–S24, 1998. View at Google Scholar · View at Scopus
  138. H. Chen, S. Shoumura, S. Emura, M. Utsumi, T. Yamahira, and H. Isono, “Effects of melatonin on the ultrastructure of the golden hamster parathyroid gland,” Histology and Histopathology, vol. 6, no. 1, pp. 1–7, 1991. View at Google Scholar · View at Scopus
  139. S. Shoumura, H. Chen, S. Emura et al., “An in vitro study on the effects of melatonin on the ultrastructure of the hamster parathyroid gland,” Histology and Histopathology, vol. 7, no. 4, pp. 715–718, 1992. View at Google Scholar · View at Scopus
  140. Y. Qiu, X. Sun, X. Qiu et al., “Decreased circulating leptin level and its association with body and bone mass in girls with adolescent idiopathic scoliosis,” Spine, vol. 32, no. 24, pp. 2703–2710, 2007. View at Publisher · View at Google Scholar · View at Scopus
  141. X. Sun, Y. Qiu, X.-S. Qiu, Z.-Z. Zhu, F. Zhu, and C.-W. Xia, “Association between circulating leptin level and anthropometric parameters in girls with adolescent idiopathic scoliosis,” National Medical Journal of China, vol. 87, no. 9, pp. 594–598, 2007. View at Google Scholar · View at Scopus
  142. J. Xu, T. Wu, Z. Zhong, C. Zhao, Y. Tang, and J. Chen, “Effect and mechanism of leptin on osteoblastic differentiation of hBMSCs,” Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi, vol. 23, no. 2, pp. 140–144, 2009. View at Google Scholar · View at Scopus
  143. S. P. Kalra, M. G. Dube, and U. T. Iwaniec, “Leptin increases osteoblast-specific osteocalcin release through a hypothalamic relay,” Peptides, vol. 30, no. 5, pp. 967–973, 2009. View at Publisher · View at Google Scholar · View at Scopus
  144. K. Wlodarski and P. Wlodarski, “Leptin as a modulator of osteogenesis,” Ortopedia Traumatologia Rehabilitacja, vol. 11, no. 1, pp. 1–6, 2009. View at Google Scholar
  145. M. I. C. Alonso-Vale, S. Andreotti, S. B. Peres et al., “Melatonin enhances leptin expression by rat adipocytes in the presence of insulin,” The American Journal of Physiology—Endocrinology and Metabolism, vol. 288, no. 4, pp. E805–E812, 2005. View at Publisher · View at Google Scholar · View at Scopus
  146. R. G. Burwell, R. K. Aujla, M. P. Grevitt et al., “Pathogenesis of adolescent idiopathic scoliosis in girls—a double neuro-osseous theory involving disharmony between two nervous systems, somatic and autonomic expressed in the spine and trunk: possible dependency on sympathetic nervous system and hormones with implications for medical therapy,” Scoliosis, vol. 4, article 24, 2009. View at Publisher · View at Google Scholar
  147. K. L. Kesling and K. A. Reinker, “Scoliosis in twins: a meta-analysis of the literature and report of six cases,” Spine, vol. 22, no. 17, pp. 2009–2015, 1997. View at Publisher · View at Google Scholar · View at Scopus
  148. L. M. Kruse, J. G. Buchan, C. A. Gurnett, and M. B. Dobbs, “Polygenic threshold model with sex dimorphism in adolescent idiopathic scoliosis: the Carter effect,” Journal of Bone and Joint Surgery, vol. 94, no. 16, pp. 1485–1491, 2012. View at Publisher · View at Google Scholar
  149. C. Brewer, S. Holloway, P. Zawalnyski, A. Schinzel, and D. Fitzpatrick, “A chromosomal deletion map of human malformations,” The American Journal of Human Genetics, vol. 63, no. 4, pp. 1153–1159, 1998. View at Publisher · View at Google Scholar · View at Scopus
  150. P. F. Giampietro, R. D. Blank, C. L. Raggio et al., “Congenital and idiopathic scoliosis: clinical and genetic aspects,” Clinical Medicine & Research, vol. 1, no. 2, pp. 125–136, 2003. View at Google Scholar · View at Scopus
  151. C. A. Wise, R. Barnes, J. Gillum, J. A. Herring, A. M. Bowcock, and M. Lovett, “Localization of susceptibility to familial idiopathic scoliosis,” Spine, vol. 25, no. 18, pp. 2372–2380, 2000. View at Publisher · View at Google Scholar · View at Scopus
  152. Y. Takahashi, M. Matsumoto, T. Karasugi et al., “Lack of association between adolescent idiopathic scoliosis and previously reported single nucleotide polymorphisms in MATN1, MTNR1B, TPH1, and IGF1 in a Japanese population,” Journal of Orthopaedic Research, vol. 29, no. 7, pp. 1055–1058, 2011. View at Publisher · View at Google Scholar · View at Scopus
  153. L. M. Nelson, K. Ward, and J. W. Ogilvie, “Genetic variants in melatonin synthesis and signaling pathway are not associated with adolescent idiopathic scoliosis,” Spine, vol. 36, no. 1, pp. 37–40, 2011. View at Publisher · View at Google Scholar · View at Scopus
  154. X. Gao, D. Gordon, D. Zhang et al., “CHD7 gene polymorphisms are associated with susceptibility to idiopathic scoliosis,” The American Journal of Human Genetics, vol. 80, no. 5, pp. 957–965, 2007. View at Publisher · View at Google Scholar · View at Scopus
  155. L. Montanaro, P. Parisini, T. Greggi et al., “Evidence of a linkage between matrilin-1 gene (MATN1) and idiopathic scoliosis,” Scoliosis, vol. 1, no. 1, article 21, 2006. View at Publisher · View at Google Scholar · View at Scopus
  156. Z. Chen, N.-L. Tang, X. Cao et al., “Promoter polymorphism of matrilin-1 gene predisposes to adolescent idiopathic scoliosis in a Chinese population,” European Journal of Human Genetics, vol. 17, no. 4, pp. 525–532, 2009. View at Publisher · View at Google Scholar
  157. B. Wang, Z.-J. Chen, Y. Qiu, and W.-J. Liu, “Decreased circulating matrilin-1 levels in adolescent idiopathic scoliosis,” Zhonghua Wai Ke Za Zhi, vol. 47, no. 21, pp. 1638–1641, 2009. View at Google Scholar · View at Scopus
  158. J. W. Bae, C.-H. Cho, W.-K. Min, and U.-K. Kim, “Associations between matrilin-1 gene polymorphisms and adolescent idiopathic scoliosis curve patterns in a Korean population,” Molecular Biology Reports, vol. 39, no. 5, pp. 5561–5567, 2012, Erratum in Molecular Biology Reports, vol. 39, no. 9, p. 9275, 2012. View at Publisher · View at Google Scholar · View at Scopus
  159. S. Ohtori, T. Koshi, M. Yamashita et al., “Surgical versus nonsurgical treatment of selected patients with discogenic low back pain: a small-sized randomized trial,” Spine, vol. 36, no. 5, pp. 347–354, 2011. View at Publisher · View at Google Scholar · View at Scopus
  160. Q. Chen, Y. Zhang, D. M. Johnson, and P. F. Goetinck, “Assembly of a novel cartilage matrix protein filamentous network: molecular basis of differential requirement of von Willebrand factor A domains,” Molecular Biology of the Cell, vol. 10, no. 7, pp. 2149–2162, 1999. View at Google Scholar · View at Scopus
  161. C. Wiberg, A. R. Klatt, R. Wagener et al., “Complexes of matrilin-1 and biglycan or decorin connect collagen VI microfibrils to both collagen II and aggrecan,” Journal of Biological Chemistry, vol. 278, no. 39, pp. 37698–37704, 2003. View at Publisher · View at Google Scholar · View at Scopus
  162. I. Kou, Y. Takahashi, T. A. Johnson et al., “Genetic variants in GPR126 are associated with adolescent idiopathic scoliosis,” Nature Genetics, vol. 45, no. 6, pp. 676–679, 2013. View at Publisher · View at Google Scholar
  163. Weizman Institute of Science, “Gene Card. The human gene compendium,” 2013, http://www.genecards.org/cgi-bin/carddisp.pl?gene=LBX1&search=LBX1.
  164. C. M. Justice, N. H. Miller, B. Marosy, J. Zhang, and A. F. Wilson, “Familial idiopathic scoliosis: evidence of an X-linked susceptibility locus,” Spine, vol. 28, no. 6, pp. 589–594, 2003. View at Publisher · View at Google Scholar · View at Scopus
  165. V. Chan, G. C. Y. Fong, K. D. K. Luk et al., “A genetic locus for adolescent idiopathic scoliosis linked to chromosome 19p13.3,” The American Journal of Human Genetics, vol. 71, no. 2, pp. 401–406, 2002. View at Publisher · View at Google Scholar · View at Scopus
  166. X.-B. Cao, Y. Qiu, and X.-S. Qiu, “FBN3 gene polymorphisms in adolescent idiopathic scoliosis patients,” Zhonghua Yi Xue Za Zhi, vol. 88, no. 43, pp. 3053–3058, 2008. View at Google Scholar · View at Scopus
  167. M. Inoue, S. Minami, Y. Nakata et al., “Association between estrogen receptor gene polymorphisms and curve severity of idiopathic scoliosis,” Spine, vol. 27, no. 21, pp. 2357–2362, 2002. View at Publisher · View at Google Scholar · View at Scopus
  168. J. Wu, Y. Qiu, L. Zhang, Q. Sun, X. Qiu, and Y. He, “Association of estrogen receptor gene polymorphisms with susceptibility to adolescent idiopathic scoliosis,” Spine, vol. 31, no. 10, pp. 1131–1136, 2006. View at Publisher · View at Google Scholar · View at Scopus
  169. N. L.-S. Tang, H.-Y. Yeung, K.-M. Lee et al., “A relook into the association of the estrogen receptor α gene (PvuII, XbaI) and adolescent idiopathic scoliosis: a study of 540 Chinese cases,” Spine, vol. 31, no. 21, pp. 2463–2468, 2006. View at Publisher · View at Google Scholar · View at Scopus
  170. D. Zhao, G.-X. Qiu, and Y.-P. Wang, “Is calmodulin 1 gene/estrogen receptor-alpha gene polymorphisms correlated with double curve pattern of adolescent idiopathic scoliosis?” Zhonghua Yi Xue Za Zhi, vol. 88, no. 35, pp. 2452–2456, 2008. View at Google Scholar · View at Scopus
  171. D. Zhao, G.-X. Qiu, Y.-P. Wang et al., “Association of calmodulin1 gene polymorphisms with susceptibility to adolescent idiopathic scoliosis,” Orthopaedic Surgery, vol. 1, no. 1, pp. 58–65, 2009. View at Google Scholar · View at Scopus
  172. H.-Q. Zhang, S.-J. Lu, M.-X. Tang et al., “Association of estrogen receptor β gene polymorphisms with susceptibility to adolescent idiopathic scoliosis,” Spine, vol. 34, no. 8, pp. 760–764, 2009. View at Publisher · View at Google Scholar · View at Scopus
  173. Y. Takahashi, M. Matsumoto, T. Karasugi et al., “Replication study of the association between adolescent idiopathic scoliosis and two estrogen receptor genes,” Journal of Orthopaedic Research, vol. 29, no. 6, pp. 834–837, 2011. View at Publisher · View at Google Scholar · View at Scopus
  174. Y. Peng, G. Liang, Y. Pei, W. Ye, A. Liang, and P. Su, “Genomic polymorphisms of G-Protein Estrogen Receptor 1 are associated with severity of adolescent idiopathic scoliosis,” International Orthopaedics, vol. 36, no. 3, pp. 671–677, 2012. View at Publisher · View at Google Scholar · View at Scopus
  175. Y. Qiu, S.-H. Mao, B.-P. Qian et al., “A promoter polymorphism of neurotrophin 3 gene is associated with curve severity and bracing effectiveness in adolescent idiopathic scoliosis,” Spine, vol. 37, no. 2, pp. 127–133, 2012. View at Publisher · View at Google Scholar · View at Scopus
  176. Y. Ogura, Y. Takahashi, I. Kou et al., “A replication study for association of 5 single nucleotide polymorphisms with curve progression of adolescent idiopathic scoliosis in Japanese patients,” Spine, vol. 38, no. 7, pp. 571–575, 2013. View at Publisher · View at Google Scholar
  177. A. M. Zaidman, M. N. Zaidman, A. V. Korel, M. A. Mikhailovsky, T. Y. Eshchenko, and E. V. Grigorjeva, “Aggrecan gene expression disorder as aetiologic factor of idiopathic scoliosis,” Studies in Health Technology and Informatics, vol. 123, pp. 14–17, 2006. View at Google Scholar · View at Scopus
  178. B. Marosy, C. M. Justice, N. Nzegwu, G. Kumar, A. F. Wilson, and N. H. Miller, “Lack of association between the aggrecan gene and familial idiopathic scoliosis,” Spine, vol. 31, no. 13, pp. 1420–1425, 2006. View at Publisher · View at Google Scholar · View at Scopus
  179. N. H. Miller, B. Mims, A. Child, D. M. Milewicz, P. Sponseller, and S. H. Blanton, “Genetic analysis of structural elastic fiber and collagen genes in familial adolescent idiopathic scoliosis,” Journal of Orthopaedic Research, vol. 14, no. 6, pp. 994–999, 1996. View at Publisher · View at Google Scholar · View at Scopus
  180. B. Marosy, C. M. Justice, C. Vu et al., “Identification of susceptibility loci for scoliosis in FIS families with triple curves,” The American Journal of Medical Genetics A, vol. 152, no. 4, pp. 846–855, 2010. View at Publisher · View at Google Scholar · View at Scopus
  181. L. Baghernajad Salehi, M. Mangino, S. de Serio et al., “Assignment of a locus for autosomal dominant idiopathic scoliosis (IS) to ohuman chromosome 17p11,” Human Genetics, vol. 111, no. 4-5, pp. 401–404, 2002. View at Publisher · View at Google Scholar · View at Scopus
  182. I.-S. Eun, W. W. Park, K. T. Suh, J. I. Kim, and J. S. Lee, “Association between osteoprotegerin gene polymorphism and bone mineral density in patients with adolescent idiopathic scoliosis,” European Spine Journal, vol. 18, no. 12, pp. 1936–1940, 2009. View at Publisher · View at Google Scholar · View at Scopus
  183. H. Wang, Z. Wu, Q. Zhuang et al., “Association study of tryptophan hydroxylase 1 and arylalkylamine n-acetyltransferase polymorphisms with adolescent idiopathic scoliosis in han chinese,” Spine, vol. 33, no. 20, pp. 2199–2203, 2008. View at Publisher · View at Google Scholar · View at Scopus
  184. L. Aulisa, P. Papaleo, E. Pola et al., “Association between IL-6 and MMP-3 gene polymorphisms and adolescent idiopathic scoliosis: a case-control study,” Spine, vol. 32, no. 24, pp. 2700–2702, 2007. View at Publisher · View at Google Scholar · View at Scopus
  185. Z. Liu, N. L. S. Tang, X.-B. Cao et al., “Lack of association between the promoter polymorphisms of MMP-3 and IL-6 genes and adolescent idiopathic scoliosis: a case-control study in a chinese han population,” Spine, vol. 35, no. 18, pp. 1701–1705, 2010. View at Publisher · View at Google Scholar · View at Scopus
  186. J. S. Lee, K. T. Suh, and I. S. Eun, “Polymorphism in interleukin-6 gene is associated with bone mineral density in patients with adolescent idiopathic scoliosis,” Journal of Bone and Joint Surgery B, vol. 92, no. 8, pp. 1118–1122, 2010. View at Publisher · View at Google Scholar · View at Scopus
  187. S. Zhou, X. S. Qiu, Z. Z. Zhu, W. F. Wu, Z. Liu, and Y. Qiu, “A single-nucleotide polymorphism rs708567 in the IL-17RC gene is associated with a susceptibility to and the curve severity of adolescent idiopathic scoliosis in a Chinese Han population: a case-control study,” Musculoskeletal Disorders, vol. 13, article 181, 2012. View at Publisher · View at Google Scholar
  188. M. Mórocz, Á. Czibula, Z. B. Grózer et al., “Association study of BMP4, IL6, Leptin, MMP3, and MTNR1B gene promoter polymorphisms and adolescent idiopathic scoliosis,” Spine, vol. 36, no. 2, pp. E123–E130, 2011. View at Publisher · View at Google Scholar · View at Scopus
  189. K. T. Suh, I.-S. Eun, and J. S. Lee, “Polymorphism in vitamin D receptor is associated with bone mineral density in patients with adolescent idiopathic scoliosis,” European Spine Journal, vol. 19, no. 9, pp. 1545–1550, 2010. View at Publisher · View at Google Scholar · View at Scopus
  190. C.-W. Xia, Y. Qiu, X. Sun et al., “Vitamin D receptor gene polymorphisms in female adolescent idiopathic scoliosis patients,” National Medical Journal of China, vol. 87, no. 21, pp. 1465–1469, 2007. View at Google Scholar · View at Scopus
  191. W.-J. Chen, Y. Qiu, F. Zhu et al., “Vitamin D receptor gene polymorphisms: no association with low bone mineral density in adolescent idiopathic scoliosis girls,” Zhonghua Wai Ke Za Zhi, vol. 46, no. 15, pp. 1183–1186, 2008. View at Google Scholar · View at Scopus
  192. Y. Takahashi, I. Kou, A. Takahashi et al., “A genome-wide association study identifies common variants near LBX1 associated with adolescent idiopathic scoliosis,” Nature Genetics, vol. 43, no. 12, pp. 1237–1240, 2011. View at Publisher · View at Google Scholar · View at Scopus
  193. W. Gao, Y. Peng, G. Liang et al., “Association between common variants near LBX1 and adolescent idiopathic scoliosis replicated in the Chinese Han population,” PLoS ONE, vol. 8, no. 1, Article ID e53234, 2013. View at Publisher · View at Google Scholar
  194. Y.-H. Fan, Y.-Q. Song, D. Chan et al., “SNP rs11190870 near LBX1 is associated with adolescent idiopathic scoliosis in southern Chinese,” Journal of Human Genetics, vol. 57, no. 4, pp. 244–246, 2012. View at Publisher · View at Google Scholar · View at Scopus
  195. S. Sharma, X. Gao, D. Londono et al., “Genome-wide association studies of adolescent idiopathic scoliosis suggest candidate susceptibility genes,” Human Molecular Genetics, vol. 20, no. 7, pp. 1456–1466, 2011. View at Publisher · View at Google Scholar · View at Scopus
  196. S. Mao, L. Xu, Z. Zhu et al., “Association between genetic determinants of peak height velocity during puberty and predisposition to adolescent idiopathic scoliosis,” Spine, vol. 38, no. 12, pp. 1034–1039, 2013. View at Publisher · View at Google Scholar
  197. T. Yang, Q. Jia, H. Guo et al., “Epidemiological survey of idiopathic scoliosis and sequence alignment analysis of multiple candidate genes,” International Orthopaedics, vol. 36, no. 6, pp. 1307–1314, 2012. View at Publisher · View at Google Scholar
  198. D.-S. Huang, G.-Y. Liang, and P.-Q. Su, “Association of matrix metalloproteinase 9 polymorphisms with adolescent idiopathic scoliosis in Chinese Han female,” Chinese Journal of Medical Genetics, vol. 28, no. 5, pp. 532–535, 2011. View at Publisher · View at Google Scholar · View at Scopus
  199. H. Wang, Z.-H. Wu, Q.-Y. Zhuang, and G.-X. Qiu, “Association study of HTR1A and HTR1B with adolescent idiopathic scoliosis,” Zhonghua Wai Ke Za Zhi, vol. 48, no. 4, pp. 296–299, 2010. View at Google Scholar · View at Scopus
  200. J. Jiang, Y. Qiu, B.-P. Qian et al., “Association between tissue inhibitor of metalloproteinase-2 gene polymorphism and adolescent idiopathic thoracic scoliosis,” Zhonghua Wai Ke Za Zhi, vol. 48, no. 6, pp. 423–426, 2010. View at Google Scholar · View at Scopus
  201. Q.-Y. Zhuang, Z.-H. Wu, and G.-X. Qiu, “Is polymorphism of CALM1 gene or growth hormone receptor gene associated with susceptibility to adolescent idiopathic scoliosis?” Chinese Medical Journal, vol. 87, no. 31, pp. 2198–2202, 2007. View at Google Scholar
  202. Axial Biotech, “ScoliScore AIS Prognostic Test,” Axial Biotech Inc., Salt Lake City, Utah, USA, 2010, http://www.axialbiotech.com/.
  203. K. Ward, J. W. Ogilvie, M. V. Singleton, R. Chettier, G. Engler, and L. M. Nelson, “Validation of DNA-based prognostic testing to predict spinal curve progression in adolescent idiopathic scoliosis,” Spine, vol. 35, no. 25, pp. E1455–E1464, 2010. View at Publisher · View at Google Scholar · View at Scopus
  204. T. B. Grivas, E. Vasiliadis, M. Malakasis, V. Mouzakis, and D. Segos, “Intervertebral disc biomechanics in the pathogenesis of idiopathic scoliosis,” Studies in Health Technology and Informatics, vol. 123, pp. 80–83, 2006. View at Google Scholar · View at Scopus
  205. H. N. Modi, S. W. Suh, H.-R. Song, J.-H. Yang, H.-J. Kim, and C. H. Modi, “Differential wedging of vertebral body and intervertebral disc in thoracic and lumbar spine in adolescent idiopathic scoliosis—a cross sectional study in 150 patients,” Scoliosis, vol. 3, no. 1, article 11, 2008. View at Publisher · View at Google Scholar · View at Scopus
  206. S. Nomura and T. Takano-Yamamoto, “Molecular events caused by mechanical stress in bone,” Matrix Biology, vol. 19, no. 2, pp. 91–96, 2000. View at Publisher · View at Google Scholar · View at Scopus
  207. A. Liedert, D. Kaspar, R. Blakytny, L. Claes, and A. Ignatius, “Signal transduction pathways involved in mechanotransduction in bone cells,” Biochemical and Biophysical Research Communications, vol. 349, no. 1, pp. 1–5, 2006. View at Publisher · View at Google Scholar · View at Scopus
  208. D. J. Papachristou, K. K. Papachroni, E. K. Basdra, and A. G. Papavassiliou, “Signaling networks and transcription factors regulating mechanotransduction in bone,” BioEssays, vol. 31, no. 7, pp. 794–804, 2009. View at Publisher · View at Google Scholar · View at Scopus
  209. K.-H. W. Lau, S. Kapur, C. Kesavan, and D. J. Baylink, “Up-regulation of the Wnt, estrogen receptor, insulin-like growth factor-I, and bone morphogenetic protein pathways in C57BL/6J osteoblasts as opposed to C3H/HeJ osteoblasts in part contributes to the differential anabolic response to fluid shear,” Journal of Biological Chemistry, vol. 281, no. 14, pp. 9576–9588, 2006. View at Publisher · View at Google Scholar · View at Scopus
  210. H. M. Frost, “A 2003 update of bone physiology and Wolff s law for clinicians,” Angle Orthodontist, vol. 74, no. 1, pp. 3–15, 2004. View at Google Scholar · View at Scopus
  211. J. Sarwark and C.-É. Aubin, “Growth considerations of the immature spine,” Journal of Bone and Joint Surgery A, vol. 89, supplement 1, pp. 8–13, 2007. View at Publisher · View at Google Scholar · View at Scopus
  212. A. Meir, D. S. McNally, J. C. Fairbank, D. Jones, and J. P. Urban, “The internal pressure and stress environment of the scoliotic intervertebral disc—a review,” Proceedings of the Institution of Mechanical Engineers H, vol. 222, no. 2, pp. 209–219, 2008. View at Publisher · View at Google Scholar · View at Scopus
  213. A. R. Meir, J. C. T. Fairbank, D. A. Jones, D. S. McNally, and J. P. G. Urban, “High pressures and asymmetrical stresses in the scoliotic disc in the absence of muscle loading,” Scoliosis, vol. 2, no. 1, article 4, 2007. View at Publisher · View at Google Scholar · View at Scopus
  214. W. E. B. Johnson and S. Roberts, “Human intervertebral disc cell morphology and cytoskeletal composition: a preliminary study of regional variations in health and disease,” Journal of Anatomy, vol. 203, no. 6, pp. 605–612, 2003. View at Publisher · View at Google Scholar · View at Scopus
  215. J. L. Ford, P. Jones, and S. Downes, “Cellularity of human annulus tissue: an investigation into the cellularity of tissue of different pathologies,” Histopathology, vol. 41, no. 6, pp. 531–537, 2002. View at Publisher · View at Google Scholar · View at Scopus
  216. H. Bertram, E. Steck, G. Zimmermann et al., “Accelerated intervertebral disc degeneration in scoliosis versus physiological ageing develops against a background of enhanced anabolic gene expression,” Biochemical and Biophysical Research Communications, vol. 342, no. 3, pp. 963–972, 2006. View at Publisher · View at Google Scholar · View at Scopus
  217. J. Antoniou, V. Arlet, T. Goswami, M. Aebi, and M. Alini, “Elevated synthetic activity in the convex side of scoliotic intervertebral discs and endplates compared with normal tissues,” Spine, vol. 26, no. 10, pp. E198–206, 2001. View at Google Scholar · View at Scopus
  218. S. Stern, K. Lindenhayn, and C. Perka, “Human intervertebral disc cell culture for disc disorders,” Clinical Orthopaedics and Related Research, no. 419, pp. 238–244, 2004. View at Google Scholar · View at Scopus
  219. J. Yu, J. C. T. Fairbank, S. Roberts, and J. P. G. Urban, “The elastic fiber network of the anulus fibrosus of the normal and scoliotic human intervertebral disc,” Spine, vol. 30, no. 16, pp. 1815–1820, 2005. View at Publisher · View at Google Scholar · View at Scopus
  220. S. Akhtar, J. R. Davies, and B. Caterson, “Ultrastructural immunolocalization of α-elastin and keratan sulfate proteoglycan in normal and scoliotic lumbar disc,” Spine, vol. 30, no. 15, pp. 1762–1769, 2005. View at Publisher · View at Google Scholar · View at Scopus
  221. S. Roberts, J. Menage, and S. M. Eisenstein, “The cartilage end-plate and intervertebral disc in scoliosis: calcification and other sequelae,” Journal of Orthopaedic Research, vol. 11, no. 5, pp. 747–757, 1993. View at Publisher · View at Google Scholar · View at Scopus
  222. L. Haglund, J. Ouellet, and P. Roughley, “Variation in chondroadherin abundance and fragmentation in the human scoliotic disc,” Spine, vol. 34, no. 14, pp. 1513–1518, 2009. View at Publisher · View at Google Scholar · View at Scopus
  223. A. Pedrini Mille, V. A. Pedrini, and C. Tudisco, “Proteoglycans of human scoliotic intervertebral disc,” Journal of Bone and Joint Surgery A, vol. 65, no. 6, pp. 815–823, 1983. View at Google Scholar · View at Scopus
  224. Y. He, Y. Qiu, F. Zhu, and Z. Zhu, “Quantitative analysis of types I and II collagen in the disc annulus in adolescent idiopathic scoliosis,” Studies in Health Technology and Informatics, vol. 123, pp. 123–128, 2006. View at Google Scholar · View at Scopus
  225. Q. Lin, Z.-H. Wu, Y. Liu et al., “Gene expression of type X collagen in the intervertebral disc of idiopathic scoliosis patients,” Zhongguo Yi Xue Ke Xue Yuan Xue Bao, vol. 26, no. 6, pp. 696–699, 2004. View at Google Scholar · View at Scopus
  226. S. Roberts, E. H. Evans, D. Kletsas, D. C. Jaffray, and S. M. Eisenstein, “Senescence in human intervertebral discs,” European Spine Journal, vol. 15, supplement 3, pp. S312–S316, 2006. View at Publisher · View at Google Scholar · View at Scopus
  227. B. Chen, J. Fellenberg, H. Wang, C. Carstens, and W. Richter, “Occurrence and regional distribution of apoptosis in scoliotic discs,” Spine, vol. 30, no. 5, pp. 519–524, 2005. View at Publisher · View at Google Scholar · View at Scopus
  228. J. K. G. Crean, S. Roberts, D. C. Jaffray, S. M. Eisenstein, and V. C. Duance, “Matrix metalloproteinases in the human intervertebral disc: role in disc degeneration and scoliosis,” Spine, vol. 22, no. 24, pp. 2877–2884, 1997. View at Publisher · View at Google Scholar · View at Scopus
  229. G. R. Buttermann and W. J. Mullin, “Pain and disability correlated with disc degeneration via magnetic resonance imaging in scoliosis patients,” European Spine Journal, vol. 17, no. 2, pp. 240–249, 2008. View at Publisher · View at Google Scholar · View at Scopus
  230. Y. Qiu and F. Zhu, “Anterior and posterior spinal growth plates in adolescent idiopathic scoliosis: a histological study,” Zhongguo Yi Xue Ke Xue Yuan Xue Bao, vol. 27, no. 2, pp. 148–152, 2005. View at Google Scholar · View at Scopus
  231. F. Zhu, Y. Qiu, H. Y. Yeung, K. M. Lee, and J. C.-Y. Cheng, “Histomorphometric study of the spinal growth plates in idiopathic scoliosis and congenital scoliosis,” Pediatrics International, vol. 48, no. 6, pp. 591–598, 2006. View at Publisher · View at Google Scholar · View at Scopus
  232. S. Wang, Y. Qiu, Z. Zhu, Z. Ma, C. Xia, and F. Zhu, “Histomorphological study of the spinal growth plates from the convex side and the concave side in adolescent idiopathic scoliosis,” Journal of Orthopaedic Surgery and Research, vol. 2, no. 1, article 19, 2007. View at Publisher · View at Google Scholar · View at Scopus
  233. H. Xu, G. Qiu, Z. Wu et al., “Expression of transforming growth factor and basic fibroblast growth factor and core protein of proteoglycan in human vertebral cartilaginous endplate of adolescent idiopathic scoliosis,” Spine, vol. 30, no. 17, pp. 1973–1978, 2005. View at Publisher · View at Google Scholar · View at Scopus
  234. G.-X. Qiu, Q.-Y. Li, Y. Liu et al., “Expression of transforming growth factor-β1 and basic fibroblast growth factor in articular process cartilages of adolescent idiopathic scoliosis,” National Medical Journal of China, vol. 86, no. 21, pp. 1478–1483, 2006. View at Google Scholar · View at Scopus
  235. Y. Chen, Y. Hu, and Z. Lü, “Regulating effects of transforming growth factor-beta (TGF-beta) on gene expression of collagen type II in human intervertebral discs,” Zhonghua Wai Ke Za Zhi, vol. 38, no. 9, pp. 703–706, 2000. View at Google Scholar · View at Scopus
  236. M. Turgut, G. Öktem, S. Uslu, M. E. Yurtseven, H. Aktuǧ, and A. Uysal, “The effect of exogenous melatonin administration on trabecular width, ligament thickness and TGF-β1 expression in degenerated intervertebral disk tissue in the rat,” Journal of Clinical Neuroscience, vol. 13, no. 3, pp. 357–363, 2006. View at Publisher · View at Google Scholar · View at Scopus
  237. E. Solheim, “Growth factors in bone,” International Orthopaedics, vol. 22, no. 6, pp. 410–416, 1998. View at Publisher · View at Google Scholar · View at Scopus
  238. H.-J. Im, P. Muddasani, V. Natarajan et al., “Basic fibroblast growth factor stimulates matrix metalloproteinase-13 via the molecular cross-talk between the mitogen-activated protein kinases and protein kinase Cδ pathways in human adult articular chondrocytes,” Journal of Biological Chemistry, vol. 282, no. 15, pp. 11110–11121, 2007. View at Publisher · View at Google Scholar · View at Scopus
  239. E. Scott Graham, D. G. Hazlerigg, and P. J. Morgan, “Evidence for regulation of basic fibroblast growth factor gene expression by photoperiod and melatonin in the ovine pars tuberalis,” Molecular and Cellular Endocrinology, vol. 156, no. 1-2, pp. 45–53, 1999. View at Publisher · View at Google Scholar · View at Scopus
  240. M.-Y. Akoume, B. Azeddine, I. Turgeon et al., “Cell-based screening test for idiopathic scoliosis using cellular dielectric spectroscopy,” Spine, vol. 35, no. 13, pp. E601–E608, 2010. View at Publisher · View at Google Scholar · View at Scopus