Nanotomography of Cell Surfaces with Evanescent Fields

Figure 1

Illumination device (microscope condenser) for variable-angle TIRFM with an additional option for transillumination and phase contrast microscopy (a) scheme, (b) experimental setup, and (c) fluorescence images of the cytoplasm marker calcein in a U373MG glioblastoma cell upon variable-angle illumination.

(a)

(b)

(c)