Nanotomography of Cell Surfaces with Evanescent Fields
Figure 2
Fluorescence images of chromosomes from T47D breast cancer cells stained
with DAPI in metaphase. (a) Conventional fluorescence microscopy, (b) TIRFM at , and (c) tomographic imaging
of chromosome-substrate distances (fluorescence excited at 391 nm and
detected at nm; image size:
55 m 55 m). The arrow marks overlapping
chromosomes.
