Nanotomography of Cell Surfaces with Evanescent Fields

Figure 3

(a) Phase contrast, (b) whole cell fluorescence, and (c) TIRFM images of U373MG glioblastoma cells upon incubation with laurdan either without further treatment (upper series) or with 30% cholesterol depletion (by 1 mM MβCD; lower series). Fluorescence excited at 391 nm and detected at 𝜆≥415 nm; image size: 200 𝜇m × 150 𝜇m. Real colour images recorded with a 3-chip CCD camera.

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