Nanotomography of Cell Surfaces with Evanescent Fields

Figure 5

TIRF image of 5-ALA induced PP IX (𝐴, Θ=66^∘) as well as (b) cell-substrate topology prior and (c) after PDT (633 nm; 4 J/cm²). Fluorescence excited at 391 nm and detected at 𝜆≥590 nm; image size: 140 𝜇m × 140 𝜇m; cell-substrate distances (colour scale) given in nanometres. Reproduced from [5] with modifications.

(a)

(b)

(c)