A Ratiometric Fluorescence Imaging System for Surgical Guidance
Figure 5
(a) Normalized PpIX fluorescence
spectra of tumor and normal contralateral brain in vivo, 4 hours after i.p. administration
of 100 mg kg−1 of ALA, and corresponding spectra in CNS-1 cells in vitro
compared to controls (no ALA). The insert shows the excitation and emission
spectra of PpIX in solution. (b)–(d) In vivo
fluorescence images of PpIX in tumor after i.p. injection of 20, 50, and 100 mg kg−1 ALA, respectively. (e) PpIX fluorescence from tumor-bearing animals 4 hours after
injection of different ALA doses in tumor and contralateral normal brain (means ±1
standard deviation: ).
