Table of Contents
Advances in Otolaryngology
Volume 2014, Article ID 415821, 7 pages
http://dx.doi.org/10.1155/2014/415821
Research Article

Evaluation of Autogenous Engineered Septal Cartilage Grafts in Rabbits: A Minimally Invasive Preclinical Model

1Division of Otolaryngology-Head and Neck Surgery, University of California, 3350 La Jolla Village Drive, 112-C, San Diego, CA 92161, USA
2Head and Neck Surgery Section, VA San Diego Healthcare System, San Diego, CA 92161, USA
3Department of Orthopedic Surgery, University of California, San Diego, La Jolla, CA 92161, USA
4Department of Bioengineering, University of California, San Diego, La Jolla, CA 92161, USA
5Creighton University School of Medicine, Omaha, NE 68102, USA
6University of North Texas Health Science Center, Fort Worth, TX 76107, USA

Received 18 April 2014; Revised 11 June 2014; Accepted 12 June 2014; Published 23 July 2014

Academic Editor: Samy Elwany

Copyright © 2014 Anton Kushnaryov et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objectives. (1) Evaluate safety of autogenous engineered septal neocartilage grafts and (2) compare properties of implanted grafts versus controls. Study Design. Prospective, basic science. Setting. Research laboratory. Methods. Constructs were fabricated from septal cartilage and then cultured in vitro or implanted on the nasal dorsum as autogenous grafts for 30 or 60 days. Rabbits were monitored for local and systemic complications. Histological, biochemical, and biomechanical properties of constructs were evaluated. Results. No serious complications were observed. Implanted constructs contained more DNA ( ) and less sGAG perDNA ( ) when compared with in vitro controls. Confined compressive aggregate moduli were also higher in implanted constructs ( ) and increased with longer in vivo incubation time ( ). Implanted constructs displayed resorption rates of 20–45 percent. Calcium deposition in implanted constructs was observe. Conclusion. Autogenous engineered septal cartilage grafts were well tolerated. As seen in experiments with athymic mice, implanted constructs accumulated more DNA and less sGAG when compared with in vitro controls. Confined compressive aggregate moduli were higher in implanted constructs. Implanted constructs displayed resorption rates similar to previously published studies using autogenous implants of native cartilage.